Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 968313..968947 Replicon chromosome
Accession NZ_CP074410
Organism Pseudomonas syringae strain U643

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag KHW15_RS04280 Protein ID WP_003345997.1
Coordinates 968313..968717 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M4XXE1
Locus tag KHW15_RS04285 Protein ID WP_003345999.1
Coordinates 968717..968947 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW15_RS04250 (KHW15_04250) 964096..964578 + 483 WP_003345989.1 PAS domain-containing protein -
KHW15_RS04255 (KHW15_04255) 964675..965931 + 1257 WP_058445621.1 3-phosphoshikimate 1-carboxyvinyltransferase -
KHW15_RS04260 (KHW15_04260) 965954..966616 - 663 WP_003408532.1 ChrR family anti-sigma-E factor -
KHW15_RS04265 (KHW15_04265) 966616..967128 - 513 WP_003345995.1 sigma-70 family RNA polymerase sigma factor -
KHW15_RS04270 (KHW15_04270) 967364..967552 - 189 WP_024663313.1 hypothetical protein -
KHW15_RS04275 (KHW15_04275) 967551..968126 + 576 WP_024663312.1 hypothetical protein -
KHW15_RS04280 (KHW15_04280) 968313..968717 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
KHW15_RS04285 (KHW15_04285) 968717..968947 - 231 WP_003345999.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHW15_RS04290 (KHW15_04290) 969064..969945 - 882 WP_044311980.1 aldose 1-epimerase -
KHW15_RS04295 (KHW15_04295) 969920..970891 - 972 WP_004402547.1 DMT family transporter -
KHW15_RS04300 (KHW15_04300) 970976..972256 - 1281 WP_003408543.1 TRAP transporter large permease -
KHW15_RS04305 (KHW15_04305) 972257..972784 - 528 WP_003408544.1 TRAP transporter small permease -
KHW15_RS04310 (KHW15_04310) 972848..973873 - 1026 WP_032658988.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 959295..968947 9652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T202055 WP_003345997.1 NZ_CP074410:c968717-968313 [Pseudomonas syringae]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T202055 NZ_CP099303:c4217748-4217464 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATAAGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8580.59 Da        Isoelectric Point: 4.5557

>AT202055 WP_003345999.1 NZ_CP074410:c968947-968717 [Pseudomonas syringae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPAEQQRESL

Download         Length: 231 bp

>AT202055 NZ_CP099303:c4217980-4217738 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SM35

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4XXE1

References