Detailed information of TA system TA202053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4322284..4322907
• Replicon: chromosome
• Accession: NZ_CP074409
• Organism: Pseudomonas syringae strain KF529

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KHW12_RS18475
• Protein ID: WP_046265609.1
• Coordinates: 4322725..4322907 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0P9PIH5
• Locus tag: KHW12_RS18470
• Protein ID: WP_025990771.1
• Coordinates: 4322284..4322688 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW12_RS18455 (KHW12_18450)	4318050..4318718	+	669	WP_024683928.1	ABC transporter ATP-binding protein	-
KHW12_RS18460 (KHW12_18455)	4318718..4321198	+	2481	WP_046265608.1	ABC transporter permease	-
KHW12_RS18465 (KHW12_18460)	4321188..4322267	+	1080	WP_032659881.1	lipocalin-like domain-containing protein	-
KHW12_RS18470 (KHW12_18465)	4322284..4322688	-	405	WP_025990771.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KHW12_RS18475 (KHW12_18470)	4322725..4322907	-	183	WP_046265609.1	type II toxin-antitoxin system HicA family toxin	Toxin
KHW12_RS18480 (KHW12_18475)	4323410..4324153	+	744	WP_249732434.1	hypothetical protein	-
KHW12_RS18485 (KHW12_18480)	4324174..4325418	-	1245	WP_213629558.1	SGNH/GDSL hydrolase family protein	-
KHW12_RS18490 (KHW12_18485)	4325415..4325594	-	180	WP_029573084.1	hypothetical protein	-
KHW12_RS18495 (KHW12_18490)	4325651..4326181	-	531	WP_213629560.1	DUF2514 domain-containing protein	-
KHW12_RS18500 (KHW12_18495)	4326178..4326711	-	534	WP_210077777.1	glycoside hydrolase family 19 protein	-
KHW12_RS18505 (KHW12_18500)	4326772..4327140	-	369	WP_116895140.1	phage tail protein	-
KHW12_RS18510 (KHW12_18505)	4327154..4327468	-	315	WP_213629562.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4322284..4387182	64898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6777.99 Da
• Isoelectric Point: 10.6643

>T202053 WP_046265609.1 NZ_CP074409:c4322907-4322725 [Pseudomonas syringae]
VQSRQLIKELEADGWVLDRVSGSHHMFKHPEKLQTVPVPHPKKDLPFGTVRAIKKLAGLI

Nucleotide

• Length: 183 bp

>T202053 NZ_CP099303:c3730569-3730201 [Citrobacter freundii]
ATGAAAACTCTACCTGCAACAATTTCGCGGGCGACGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGATGCTAAGCGACACACCTTTTAGTGATGAAACCATTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGCTGATGAGTCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGTCGATCACGGCTGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14455.50 Da
• Isoelectric Point: 5.1546

>AT202053 WP_025990771.1 NZ_CP074409:c4322688-4322284 [Pseudomonas syringae]
MKYPMCIEWGNDTTAFGIQIPDIPGAVTAGDTFEEAHAAAVEIAHIMLQEIAASGGTIPKAGNVAEHARNPDFAGMGWGM
IEIDVTPYLGKTEKVNVTLPGFVIRQIDRYVRDHSIKSRSTFLADAALEKLGRA

Nucleotide

• Length: 405 bp

>AT202053 NZ_CP099303:c3730907-3730590 [Citrobacter freundii]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAAGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATCACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAAATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGCGTCATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGACACTCTTGGCAGTTGCGGCTATGTATACATCTCCGTCTATCCCACTCAACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A0P9PIH5