Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RHH
Location 1843937..1844450 Replicon chromosome
Accession NZ_CP074409
Organism Pseudomonas syringae strain KF529

Toxin (Protein)


Gene name relE Uniprot ID A0A0P9GC66
Locus tag KHW12_RS08170 Protein ID WP_003423365.1
Coordinates 1843937..1844221 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A3M4E5F0
Locus tag KHW12_RS08175 Protein ID WP_024648170.1
Coordinates 1844211..1844450 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW12_RS08155 (KHW12_08155) 1840079..1842007 + 1929 WP_016567635.1 methyl-accepting chemotaxis protein -
KHW12_RS08160 (KHW12_08160) 1842041..1842973 - 933 WP_213630692.1 DMT family transporter -
KHW12_RS08165 (KHW12_08165) 1843048..1843902 + 855 WP_003423353.1 LysR family transcriptional regulator -
KHW12_RS08170 (KHW12_08170) 1843937..1844221 - 285 WP_003423365.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KHW12_RS08175 (KHW12_08175) 1844211..1844450 - 240 WP_024648170.1 CopG family ribbon-helix-helix protein Antitoxin
KHW12_RS08180 (KHW12_08180) 1844609..1845625 - 1017 WP_010425855.1 1-aminocyclopropane-1-carboxylate deaminase -
KHW12_RS08185 (KHW12_08185) 1845812..1846318 + 507 WP_003315203.1 Lrp/AsnC family transcriptional regulator -
KHW12_RS08190 (KHW12_08190) 1846465..1847373 + 909 WP_003415812.1 DUF808 domain-containing protein -
KHW12_RS08195 (KHW12_08195) 1847550..1848335 - 786 WP_003396313.1 outer membrane protein OmpK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-89)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-41)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10632.34 Da        Isoelectric Point: 10.4249

>T202052 WP_003423365.1 NZ_CP074409:c1844221-1843937 [Pseudomonas syringae]
MQVEWLKTALKNLDEEAAYIALDNPAAAAAFVKAIQSSVTQLASFPAMGREGRIAGTREWPLPDLPYLIPYRIRSGRLQV
LRIFHTRRQSPPVW

Download         Length: 285 bp

>T202052 NZ_CP099303:3520701-3520919 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 8715.80 Da        Isoelectric Point: 4.4354

>AT202052 WP_024648170.1 NZ_CP074409:c1844450-1844211 [Pseudomonas syringae]
MSTMSLRLPDEMADTLAHLAKATGRSKSFLALDALREYLTREAWQIAEIQRAIEEADAGEFASQEDVKAVMDKWSGNAG

Download         Length: 240 bp

>AT202052 NZ_CP099303:3520299-3520673 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0P9GC66

Antitoxin

Source ID Structure
AlphaFold DB A0A3M4E5F0

References