Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 931196..931830 Replicon chromosome
Accession NZ_CP074409
Organism Pseudomonas syringae strain KF529

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag KHW12_RS04155 Protein ID WP_003345997.1
Coordinates 931196..931600 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0P9J133
Locus tag KHW12_RS04160 Protein ID WP_003421652.1
Coordinates 931600..931830 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW12_RS04125 (KHW12_04125) 926979..927461 + 483 WP_003345989.1 PAS domain-containing protein -
KHW12_RS04130 (KHW12_04130) 927558..928814 + 1257 WP_004402530.1 3-phosphoshikimate 1-carboxyvinyltransferase -
KHW12_RS04135 (KHW12_04135) 928837..929499 - 663 WP_003408532.1 ChrR family anti-sigma-E factor -
KHW12_RS04140 (KHW12_04140) 929499..930011 - 513 WP_003345995.1 sigma-70 family RNA polymerase sigma factor -
KHW12_RS04145 (KHW12_04145) 930247..930435 - 189 WP_024665471.1 hypothetical protein -
KHW12_RS04150 (KHW12_04150) 930434..931009 + 576 WP_024663312.1 hypothetical protein -
KHW12_RS04155 (KHW12_04155) 931196..931600 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
KHW12_RS04160 (KHW12_04160) 931600..931830 - 231 WP_003421652.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHW12_RS04165 (KHW12_04165) 931947..932828 - 882 WP_116817130.1 aldose 1-epimerase -
KHW12_RS04170 (KHW12_04170) 932803..933774 - 972 Protein_819 DMT family transporter -
KHW12_RS04175 (KHW12_04175) 933859..935139 - 1281 WP_003421655.1 TRAP transporter large permease -
KHW12_RS04180 (KHW12_04180) 935140..935667 - 528 WP_003408544.1 TRAP transporter small permease -
KHW12_RS04185 (KHW12_04185) 935731..936756 - 1026 WP_096113444.1 TRAP transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(7-34)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T202051 WP_003345997.1 NZ_CP074409:c931600-931196 [Pseudomonas syringae]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T202051 NZ_CP099303:c2360054-2359776 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAGGGATTGCGAGATTTGTTTCTTCATGGAAGAACATCAGGCGTTATGGCGAAACATGT
CCGACGATTACGACACAGGCTTGCCGTTATTGATGCGGCAAGCAAGGTTACAGATATCAATATGCCCGGTTATAAGCTAC
ATCCTCTGGTAGGTGACCGTGATGGCATATGGGCAATAACTGTTTCAGGTAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGACGCGTACATACTGGACTACGAGGACTATCATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8508.52 Da        Isoelectric Point: 4.7247

>AT202051 WP_003421652.1 NZ_CP074409:c931830-931600 [Pseudomonas syringae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAERGQPAEQQRESL

Download         Length: 231 bp

>AT202051 NZ_CP099303:c2359776-2359492 [Citrobacter freundii]
ATGAAGATGGCAAATCATCCCCGTCCTGGGGATATCATTCAGGAAGCGCTGGAAGAACTCAATGTCAGCCTGCGCGAGTT
TGCCAGAGCTATGGAGATTGCACCTTCAACGGCAAGTCGACTATTAACCGGGAAAGCAGCCCTAACGCCTGAGATGGCGA
TTAAACTCTCTGTGGTGATTGGCAGCTCACCCGAAATGTGGTTAAACCTGCAAACTACCTGGAGTCTGGCAGAGGCCCAA
AAAAGCGTGGATATTTCACGCCTGCGTAAGCTGGCAGTACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A193SM35

Antitoxin

Source ID Structure
AlphaFold DB A0A0P9J133

References