Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4905128..4905746 Replicon chromosome
Accession NZ_CP074407
Organism Escherichia coli strain GDAR2-2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KHW16_RS23735 Protein ID WP_001291435.1
Coordinates 4905528..4905746 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KHW16_RS23730 Protein ID WP_000344800.1
Coordinates 4905128..4905502 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW16_RS23720 (4900217) 4900217..4901410 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHW16_RS23725 (4901433) 4901433..4904582 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KHW16_RS23730 (4905128) 4905128..4905502 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KHW16_RS23735 (4905528) 4905528..4905746 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KHW16_RS23740 (4905917) 4905917..4906468 + 552 WP_000102564.1 maltose O-acetyltransferase -
KHW16_RS23745 (4906584) 4906584..4907054 + 471 WP_000136192.1 YlaC family protein -
KHW16_RS23750 (4907218) 4907218..4908768 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KHW16_RS23755 (4908810) 4908810..4909163 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KHW16_RS23765 (4909542) 4909542..4909853 + 312 WP_000409911.1 MGMT family protein -
KHW16_RS23770 (4909884) 4909884..4910456 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202044 WP_001291435.1 NZ_CP074407:4905528-4905746 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T202044 NZ_CP099298:c4520234-4520016 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT202044 WP_000344800.1 NZ_CP074407:4905128-4905502 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT202044 NZ_CP099298:c4520636-4520262 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References