Detailed information of TA system TA202042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3732573..3734161
• Replicon: chromosome
• Accession: NZ_CP074407
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F4NFZ0
• Locus tag: KHW16_RS18155
• Protein ID: WP_001125463.1
• Coordinates: 3732839..3734161 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KHW16_RS18150
• Protein ID: WP_001296726.1
• Coordinates: 3732573..3732839 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS18150 (3732573)	3732573..3732839	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
KHW16_RS18155 (3732839)	3732839..3734161	+	1323	WP_001125463.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
KHW16_RS18160 (3734473)	3734473..3734681	+	209	Protein_3545	AraC family transcriptional regulator	-
KHW16_RS18165 (3735115)	3735115..3735678	+	564	WP_001296758.1	type 1 fimbrial major subunit FimA	-
KHW16_RS18170 (3736039)	3736039..3736749	+	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimA	3728364..3748663	20299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49422.05 Da
• Isoelectric Point: 9.1203

>T202042 WP_001125463.1 NZ_CP074407:3732839-3734161 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T202042 NZ_CP099298:c3065757-3065425 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGTGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGATGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT202042 WP_001296726.1 NZ_CP074407:3732573-3732839 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT202042 NZ_CP099298:c3066015-3065758 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9IYX0