Detailed information of TA system TA202031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2618239..2618864
• Replicon: chromosome
• Accession: NZ_CP074407
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KHW16_RS12505
• Protein ID: WP_000911330.1
• Coordinates: 2618466..2618864 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KHW16_RS12500
• Protein ID: WP_000450524.1
• Coordinates: 2618239..2618466 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS12475 (2614042)	2614042..2614512	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KHW16_RS12480 (2614512)	2614512..2615084	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KHW16_RS12485 (2615230)	2615230..2616108	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KHW16_RS12490 (2616125)	2616125..2617159	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
KHW16_RS12495 (2617372)	2617372..2618085	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KHW16_RS12500 (2618239)	2618239..2618466	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KHW16_RS12505 (2618466)	2618466..2618864	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KHW16_RS12510 (2619011)	2619011..2619874	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
KHW16_RS12515 (2619889)	2619889..2621904	+	2016	WP_047086710.1	tRNA cytosine(34) acetyltransferase TmcA	-
KHW16_RS12520 (2621978)	2621978..2622676	+	699	WP_000679812.1	esterase	-
KHW16_RS12525 (2622786)	2622786..2622986	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T202031 WP_000911330.1 NZ_CP074407:2618466-2618864 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T202031 NZ_CP099291:c4688217-4687858 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAACAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT202031 WP_000450524.1 NZ_CP074407:2618239-2618466 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT202031 NZ_CP099291:c4687868-4687551 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33