Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2281259..2281842 Replicon chromosome
Accession NZ_CP074407
Organism Escherichia coli strain GDAR2-2

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KHW16_RS10765 Protein ID WP_000254745.1
Coordinates 2281507..2281842 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KHW16_RS10760 Protein ID WP_000581937.1
Coordinates 2281259..2281507 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW16_RS10750 (2277598) 2277598..2278899 + 1302 WP_000046836.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KHW16_RS10755 (2278947) 2278947..2281181 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KHW16_RS10760 (2281259) 2281259..2281507 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KHW16_RS10765 (2281507) 2281507..2281842 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KHW16_RS10770 (2281913) 2281913..2282704 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KHW16_RS10775 (2282932) 2282932..2284569 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KHW16_RS10780 (2284657) 2284657..2285955 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T202030 WP_000254745.1 NZ_CP074407:2281507-2281842 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T202030 NZ_CP099291:c4431703-4431419 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT202030 WP_000581937.1 NZ_CP074407:2281259-2281507 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT202030 NZ_CP099291:c4431935-4431693 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References