Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1561119..1561705 Replicon chromosome
Accession NZ_CP074407
Organism Escherichia coli strain GDAR2-2

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KHW16_RS07210 Protein ID WP_042014162.1
Coordinates 1561119..1561349 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KHW16_RS07215 Protein ID WP_000593555.1
Coordinates 1561346..1561705 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW16_RS07200 (1557156) 1557156..1559891 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
KHW16_RS07205 (1559891) 1559891..1561015 + 1125 WP_001314210.1 ABC transporter permease -
KHW16_RS07210 (1561119) 1561119..1561349 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KHW16_RS07215 (1561346) 1561346..1561705 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KHW16_RS07220 (1561825) 1561825..1562226 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KHW16_RS07225 (1562232) 1562232..1563038 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KHW16_RS07230 (1563035) 1563035..1563799 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KHW16_RS07235 (1563799) 1563799..1564632 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KHW16_RS07240 (1564629) 1564629..1565573 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T202027 WP_042014162.1 NZ_CP074407:1561119-1561349 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T202027 NZ_CP099291:3746666-3746884 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT202027 WP_000593555.1 NZ_CP074407:1561346-1561705 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT202027 NZ_CP099291:3746264-3746638 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References