Detailed information of TA system TA202022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 10423..11260
• Replicon: chromosome
• Accession: NZ_CP074407
• Organism: Escherichia coli strain GDAR2-2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: KHW16_RS00060
• Protein ID: WP_063120031.1
• Coordinates: 10718..11260 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KHW16_RS00055
• Protein ID: WP_001297137.1
• Coordinates: 10423..10734 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW16_RS00030 (5443)	5443..6390	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KHW16_RS00035 (6412)	6412..8403	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KHW16_RS00040 (8393)	8393..9007	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KHW16_RS00045 (9007)	9007..9336	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KHW16_RS00050 (9348)	9348..10238	+	891	WP_000971336.1	heme o synthase	-
KHW16_RS00055 (10423)	10423..10734	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KHW16_RS00060 (10718)	10718..11260	+	543	WP_063120031.1	GNAT family N-acetyltransferase	Toxin
KHW16_RS00065 (11316)	11316..12251	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KHW16_RS00070 (12658)	12658..14022	+	1365	WP_001000978.1	MFS transporter	-
KHW16_RS00075 (14150)	14150..14641	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KHW16_RS00080 (14809)	14809..15720	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19705.88 Da
• Isoelectric Point: 8.0330

>T202022 WP_063120031.1 NZ_CP074407:10718-11260 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVAGKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T202022 NZ_CP099291:34610-34972 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT202022 WP_001297137.1 NZ_CP074407:10423-10734 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT202022 NZ_CP099291:34959-35258 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3