Detailed information of TA system TA202018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1074454..1075037
• Replicon: chromosome
• Accession: NZ_CP074404
• Organism: Cellulomonas fengjieae strain zg-ZUI188

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KG102_RS04935
• Protein ID: WP_208289513.1
• Coordinates: 1074693..1075037 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KG102_RS04930
• Protein ID: WP_208289407.1
• Coordinates: 1074454..1074699 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG102_RS04925 (KG102_04925)	1072042..1074336	-	2295	WP_208289408.1	UvrD-helicase domain-containing protein	-
KG102_RS04930 (KG102_04930)	1074454..1074699	+	246	WP_208289407.1	ribbon-helix-helix protein, CopG family	Antitoxin
KG102_RS04935 (KG102_04935)	1074693..1075037	+	345	WP_208289513.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KG102_RS04940 (KG102_04940)	1075374..1076531	+	1158	WP_208289405.1	class I SAM-dependent methyltransferase	-
KG102_RS04945 (KG102_04945)	1076583..1077338	-	756	WP_208214537.1	FadR/GntR family transcriptional regulator	-
KG102_RS04950 (KG102_04950)	1077548..1078486	+	939	WP_208289404.1	isoaspartyl peptidase/L-asparaginase	-
KG102_RS04955 (KG102_04955)	1078483..1079439	+	957	WP_208289403.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1063023..1075181	12158

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12070.92 Da
• Isoelectric Point: 8.6196

>T202018 WP_208289513.1 NZ_CP074404:1074693-1075037 [Cellulomonas fengjieae]
MVIERGGLYWADLGAPVGSRPAKRRPVMVISADTYNQSRLATVVALVLTSNTDLATMPGNVFLPAAATGLPRDSVANVTA
VVTLNKTDLAEQTGHTPLALMREIDRGLRGSLDL

Nucleotide

• Length: 345 bp

>T202018 NZ_CP099260:c4661340-4660807 [Klebsiella aerogenes]
ATGGATCAGCTACTGACGATTGAGATGATTGCCGATGAGTTCCGCTACGACATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAACATCTCAAGCGACAGCACGATGGACAGATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCATGCTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGCGGCATGCTGCCGTCTAAAACTCAG
CAAAAGAAAATTCCGTATCAAAATGCGCCAAGCGTAACTCTGGGGCGGCTGGCCATTGATAAATCCGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTCGCCCATGCGATGCGAGTGGTGTGGGGGGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCTCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGATGATGAGTCATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8765.56 Da
• Isoelectric Point: 4.1852

>AT202018 WP_208289407.1 NZ_CP074404:1074454-1074699 [Cellulomonas fengjieae]
VKTAISIPDETFEKASRRAHDLGMSRSEFFTRAAAHYLDELDAESVTRQIDAAVDALGVDDSAADAVAAGRRLLDAEADD
W

Nucleotide

• Length: 246 bp

>AT202018 NZ_CP099260:c4661617-4661351 [Klebsiella aerogenes]
ATGCCCGCACTCAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACTCAACCGCCAGCACCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGATAA