Detailed information of TA system TA202017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 484160..484707
• Replicon: plasmid pDG3-1g
• Accession: NZ_CP074400
• Organism: Agrobacterium salinitolerans strain DG3-1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KHC17_RS28800
• Protein ID: WP_217006170.1
• Coordinates: 484160..484456 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: KHC17_RS28805
• Protein ID: WP_217006172.1
• Coordinates: 484462..484707 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHC17_RS28780 (KHC17_28780)	479627..481039	+	1413	WP_217006166.1	amino acid permease	-
KHC17_RS28785 (KHC17_28785)	481023..481841	+	819	WP_217006168.1	methyltransferase domain-containing protein	-
KHC17_RS28790 (KHC17_28790)	482179..482634	-	456	Protein_482	IS5 family transposase	-
KHC17_RS29900	483169..483624	+	456	Protein_483	DUF6088 family protein	-
KHC17_RS29905	483617..483730	+	114	Protein_484	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KHC17_RS28795 (KHC17_28795)	483729..484115	+	387	Protein_485	hypothetical protein	-
KHC17_RS28800 (KHC17_28800)	484160..484456	-	297	WP_217006170.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHC17_RS28805 (KHC17_28805)	484462..484707	-	246	WP_217006172.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
KHC17_RS28810 (KHC17_28810)	485000..485899	-	900	WP_217006174.1	LysR family transcriptional regulator	-
KHC17_RS28815 (KHC17_28815)	486055..487515	+	1461	WP_217006176.1	argininosuccinate lyase	-
KHC17_RS28820 (KHC17_28820)	487512..488384	+	873	WP_217006178.1	amino acid ABC transporter permease	-
KHC17_RS28825 (KHC17_28825)	488395..489165	+	771	WP_149148578.1	amino acid ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..521982	521982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-92)

Antitoxin

• Antitoxin ParD (6-63)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11364.80 Da
• Isoelectric Point: 6.1304

>T202017 WP_217006170.1 NZ_CP074400:c484456-484160 [Agrobacterium salinitolerans]
MRYVLSAEAEEDIIGIAEYGVRNFGTTHARRYHNELFETLDLIARHPGMARERREIQPPVRIHPFKAHLIVYVLGEDGVV
FVIRVRHGHEDWASSDDD

Nucleotide

• Length: 297 bp

>T202017 NZ_CP099260:4200295-4200663 [Klebsiella aerogenes]
ATGAAAAATCCACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATCG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATCGATCGCAATGGATTT
ACCTCTCCGGTACAAGCACCATATTTGACTACGACAGACATTCTTCAAGCTAGGAAAGCGTGCGGGCTGATGAGCCGGTG
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCAAGGCTGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9017.09 Da
• Isoelectric Point: 7.0114

>AT202017 WP_217006172.1 NZ_CP074400:c484707-484462 [Agrobacterium salinitolerans]
MATMNVSLPNPMKEWVETQAKTGRYSNASDYVRDLIRRDQMRADKVAAMQRHVDEGIASGTGNRTSDELFKVAAARAEVS
D

Nucleotide

• Length: 246 bp

>AT202017 NZ_CP099260:4199918-4200274 [Klebsiella aerogenes]
ATGAACAATCACTCAGAATCTGGTGACAAGCCGGAGAATCCAGCCTGCCAGCAGTGGGGGCTAAAGAGTACGATCACACC
CTGTTTTGGAGCTCGTCTGGTGCAGGAAGGCAACCGGGTGCATTTTCTCGCTGACCGGGCCGGATTCAACGGTGCTTTCA
GCGACGATGACGCGCTGCACCTGGATCAGGTCTTTCCGTTGATACTCAAACAACTGGAGCTGATGCTCACCAGCGGAGAA
CTCTCTTCCCGGCATCAGCACTGCGTCACGCTATATCACAACGGACTCACGTGCGAAGCCGATACCCTTGGTAGTTGCGG
CTACGTATACATCGCCATTTATCCCACTCAACGCTAA