Detailed information of TA system TA202015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 258942..259540
• Replicon: plasmid pDG3-1g
• Accession: NZ_CP074400
• Organism: Agrobacterium salinitolerans strain DG3-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KHC17_RS27665
• Protein ID: WP_116494805.1
• Coordinates: 259247..259540 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KHC17_RS27660
• Protein ID: WP_217005875.1
• Coordinates: 258942..259250 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHC17_RS27645 (KHC17_27645)	254475..255425	-	951	WP_217005869.1	LysR family transcriptional regulator	-
KHC17_RS27650 (KHC17_27650)	255529..256650	-	1122	WP_217005871.1	serine hydrolase	-
KHC17_RS27655 (KHC17_27655)	256697..258346	-	1650	WP_217005873.1	tannase/feruloyl esterase family alpha/beta hydrolase	-
KHC17_RS27660 (KHC17_27660)	258942..259250	+	309	WP_217005875.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KHC17_RS27665 (KHC17_27665)	259247..259540	+	294	WP_116494805.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHC17_RS27670 (KHC17_27670)	259634..260515	-	882	WP_112360104.1	AraC family transcriptional regulator	-
KHC17_RS27675 (KHC17_27675)	260652..261395	+	744	WP_217005878.1	SDR family oxidoreductase	-
KHC17_RS27680 (KHC17_27680)	261410..261805	+	396	WP_217005880.1	VOC family protein	-
KHC17_RS27685 (KHC17_27685)	261807..262787	+	981	WP_217005882.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
KHC17_RS27690 (KHC17_27690)	262798..263100	+	303	WP_217005884.1	nuclear transport factor 2 family protein	-
KHC17_RS27695 (KHC17_27695)	263229..263699	+	471	WP_217005886.1	nuclear transport factor 2 family protein	-
KHC17_RS29840	263760..263834	-	75	Protein_259	short-chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..521982	521982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10708.37 Da
• Isoelectric Point: 7.0092

>T202015 WP_116494805.1 NZ_CP074400:259247-259540 [Agrobacterium salinitolerans]
MKLTWSAFALSDRDAIFTYIEADNPAAAILIDERIVAAARRLLDFPASGRVGRIAGTRELVINGTPYVAAYLITQTTVRI
LRVLHGAQEWPEQLPLS

Nucleotide

• Length: 294 bp

>T202015 NZ_CP099260:2618655-2619140 [Klebsiella aerogenes]
ATGGGAAAAATAAGCGCGCCGACGCCGCTATCGTCACACCATCAGATCGCGGAATTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTGAAACAGCGAGGATTGAAAAACCAGGCACAGGGCGCCGCGAGAACCTTTGTAGTCTGCAAGGAAGAGAGCC
ACCAGGTGGTGGGGTTTTATTCGTTAGCAACCGGCAGCGTTAATCACACGGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTATCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTCTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTTCGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCCACCACCCAGGAACGCACACTTTTCCTCGCGTTGCCT
AAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11098.58 Da
• Isoelectric Point: 5.1570

>AT202015 WP_217005875.1 NZ_CP074400:258942-259250 [Agrobacterium salinitolerans]
MAANALVQTRIDSEIRDRASAVLESMGLTVSDAVRIMLTRTANEGALPLELLSGSEAHDAWFRTKVLEALNDTRPDLPDD
EVEAHFATRRAAARLKAGLPKT

Nucleotide

• Length: 309 bp

>AT202015 NZ_CP099260:2618401-2618667 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGGGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCGCGGACCGACTTTATCCTTGAGATGGCCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGCCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA