Detailed information of TA system TA202014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 85538..87023
• Replicon: plasmid pDG3-1d
• Accession: NZ_CP074397
• Organism: Agrobacterium salinitolerans strain DG3-1

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: KHC17_RS25805
• Protein ID: WP_217005407.1
• Coordinates: 85538..86419 (-)
• Length: 294 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: KHC17_RS25810
• Protein ID: WP_217005409.1
• Coordinates: 86412..87023 (-)
• Length: 204 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHC17_RS25785 (KHC17_25785)	80598..81689	+	1092	WP_217005399.1	RHE_PE00001 family protein	-
KHC17_RS25790 (KHC17_25790)	81762..82097	+	336	WP_217005401.1	hypothetical protein	-
KHC17_RS25795 (KHC17_25795)	82094..84232	+	2139	WP_217005403.1	ATP-binding protein	-
KHC17_RS25800 (KHC17_25800)	84233..85534	+	1302	WP_217005405.1	hypothetical protein	-
KHC17_RS25805 (KHC17_25805)	85538..86419	-	882	WP_217005407.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KHC17_RS25810 (KHC17_25810)	86412..87023	-	612	WP_217005409.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
KHC17_RS25815 (KHC17_25815)	87149..87613	-	465	WP_217005411.1	very short patch repair endonuclease	-
KHC17_RS25820 (KHC17_25820)	87690..89300	+	1611	WP_217005413.1	DNA cytosine methyltransferase	-
KHC17_RS25825 (KHC17_25825)	89290..90801	+	1512	WP_217005415.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..147126	147126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-260)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (9-55)

Sequences

Toxin

Protein

• Length: 294 a.a.
• Molecular weight: 32813.58 Da
• Isoelectric Point: 5.0949

>T202014 WP_217005407.1 NZ_CP074397:c86419-85538 [Agrobacterium salinitolerans]
MAREIRNRAASIKQQLSNLARREGRVFEIVMVRYALERLLYRLSVSDKADRFILKGGMLVTLWLDGDNRETRDVDFLGYG
QADGETLRSIFAAIMAAQYDDGLVFDVGGLQAEVIREDMEYNGVRLRTVALLERTRIPVTIDIGFGDALTGAEMTSYPSL
LGMESPRIRSYPPAVVIAEKFQAMVQLGIINGRMKDYFDLWAIPQVVSIPDRELDAAIRATFERRRTPIPSGRPPGLSGV
FFDDTGKQTQWRNYARSIQVTNLDLGEVTDAIWTLVGPSCARLIAESGSDKEI

Nucleotide

• Length: 882 bp

>T202014 NZ_CP099260:1857094-1857197 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 204 a.a.
• Molecular weight: 22389.90 Da
• Isoelectric Point: 10.1394

>AT202014 WP_217005409.1 NZ_CP074397:c87023-86412 [Agrobacterium salinitolerans]
VAAATQNDRLLKYIAEHPIVRAYELRRAGIFTMTIARAVEAGELVRLSRGLYQRPDADLDTRQALAEAAKLVPQGVVALT
SALAFHGLTDQIPREVWMAVSQKDWAVAPAYPPIRITEFKDAYMKLGVEHHQISGIEVRVFSVTKSLADVFRSRLVDRSV
AIEALRAALQHRKASPAAITETAMANGAWKKMKPYLEALTSNG

Nucleotide

• Length: 612 bp

>AT202014 NZ_CP099260:c1857205-1857059 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT