Detailed information of TA system TA202012

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 597349..597956
• Replicon: chromosome
• Accession: NZ_CP074393
• Organism: Agrobacterium salinitolerans strain DG3-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KHC17_RS12525
• Protein ID: WP_217004406.1
• Coordinates: 597349..597684 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KHC17_RS12530
• Protein ID: WP_077982683.1
• Coordinates: 597681..597956 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHC17_RS12510 (KHC17_12510)	593383..595575	-	2193	WP_217004405.1	transglycosylase domain-containing protein	-
KHC17_RS12515 (KHC17_12515)	595829..596320	+	492	WP_077982686.1	YcgN family cysteine cluster protein	-
KHC17_RS12520 (KHC17_12520)	596490..597314	+	825	WP_077982685.1	class D beta-lactamase	-
KHC17_RS12525 (KHC17_12525)	597349..597684	-	336	WP_217004406.1	type II toxin-antitoxin system YafQ family toxin	Toxin
KHC17_RS12530 (KHC17_12530)	597681..597956	-	276	WP_077982683.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KHC17_RS12535 (KHC17_12535)	598064..598672	+	609	WP_217004846.1	class I SAM-dependent methyltransferase	-
KHC17_RS12540 (KHC17_12540)	598848..601430	+	2583	WP_217004407.1	heavy metal translocating P-type ATPase	-
KHC17_RS12545 (KHC17_12545)	601427..601849	+	423	WP_142912433.1	Cu(I)-responsive transcriptional regulator	-
KHC17_RS12550 (KHC17_12550)	601903..602103	+	201	WP_173988436.1	heavy-metal-associated domain-containing protein	-
KHC17_RS12555 (KHC17_12555)	602172..602438	+	267	WP_127758200.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
KHC17_RS12560 (KHC17_12560)	602438..602797	+	360	WP_142912434.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (23-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-86)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12901.74 Da
• Isoelectric Point: 8.9995

>T202012 WP_217004406.1 NZ_CP074393:c597684-597349 [Agrobacterium salinitolerans]
VTDKKGHGKDAASKRTVLPRSSDFTRQFIKDWQRLNNSGRYDMVKLKEIMLLLIAGEAPLPAQFRDHELNGEWHDHRECH
VGGDFLLIYKLDEKQNLLIFTRAGTHAELFR

Nucleotide

• Length: 336 bp

>T202012 NZ_CP099260:339581-339928 [Klebsiella aerogenes]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGCAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTTATCCTGTCTGAGTTTGGTCCACAGCTTGGGCGTCCGTATGTTGATACGGTAAAAGGTTCAAAGTTTCAGA
ATATGAAAGAACTTCGGGTTCAGCATCATGGGCTCCCAGTCCGCGCCTTTTTTGCTTTTGATCCTCTTCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACCCTGGCTGATAGAGAATT
CAGCCAGCACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9931.40 Da
• Isoelectric Point: 6.3275

>AT202012 WP_077982683.1 NZ_CP074393:c597956-597681 [Agrobacterium salinitolerans]
MTANAYVRARIDQALKDDATAVLDSLGLTVSDVMRMMLTRIAREKALPIELTQPNAETLAAIEEARAIAAARRNRFGTAE
ALFDALDGNKR

Nucleotide

• Length: 276 bp

>AT202012 NZ_CP099260:339928-340227 [Klebsiella aerogenes]
ATGGGCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGCGAAGAGTTACAAATTTCACAAACGGAGATGGCTGCGGCTATGGGGGTTAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCGAGGCTTTCGACCCTAAAGCGCTACATTGCGGCCTTGGGT
GGGGAACTAAGTATAAATGTCACGTTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA