Detailed information of TA system TA202011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 348554..349034
• Replicon: chromosome
• Accession: NZ_CP074393
• Organism: Agrobacterium salinitolerans strain DG3-1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: KHC17_RS11300
• Protein ID: WP_137409826.1
• Coordinates: 348554..348823 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: KHC17_RS11305
• Protein ID: WP_077983309.1
• Coordinates: 348807..349034 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHC17_RS11270 (KHC17_11270)	343618..344394	-	777	WP_137409822.1	L,D-transpeptidase	-
KHC17_RS11275 (KHC17_11275)	344630..345259	+	630	WP_217004370.1	DNA-3-methyladenine glycosylase I	-
KHC17_RS11280 (KHC17_11280)	345256..345699	+	444	WP_137409823.1	hypothetical protein	-
KHC17_RS11285 (KHC17_11285)	345715..346758	-	1044	WP_089153242.1	YeiH family protein	-
KHC17_RS11290 (KHC17_11290)	346840..347757	+	918	WP_137409824.1	LysR substrate-binding domain-containing protein	-
KHC17_RS11295 (KHC17_11295)	347783..348547	-	765	WP_137409825.1	hypothetical protein	-
KHC17_RS11300 (KHC17_11300)	348554..348823	-	270	WP_137409826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHC17_RS11305 (KHC17_11305)	348807..349034	-	228	WP_077983309.1	DUF6290 family protein	Antitoxin
KHC17_RS11310 (KHC17_11310)	349271..350794	+	1524	WP_137409827.1	histidine--tRNA ligase	-
KHC17_RS11315 (KHC17_11315)	350799..351167	+	369	WP_137409828.1	VOC family protein	-
KHC17_RS11320 (KHC17_11320)	351184..352311	+	1128	WP_137409829.1	ATP phosphoribosyltransferase regulatory subunit	-
KHC17_RS11325 (KHC17_11325)	352308..353000	+	693	WP_137409830.1	ATP phosphoribosyltransferase	-
KHC17_RS11330 (KHC17_11330)	353059..353505	-	447	WP_137409831.1	DoxX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-88)

Antitoxin

• Protein of unknown function DUF6290 (6-73)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10713.34 Da
• Isoelectric Point: 9.8259

>T202011 WP_137409826.1 NZ_CP074393:c348823-348554 [Agrobacterium salinitolerans]
VIWTIEYHTLVQKEMRKINPEVRRRIRSFLHERPAALDDPRQTGAALQGSELGNYWRYRVGDYRIICDIQDHKLVVLVVE
IGHRREIYR

Nucleotide

• Length: 270 bp

>T202011 NZ_CP099260:264281-264556 [Klebsiella aerogenes]
ATGGAGCAGCAGGTTTTATCTTTACGTAAAAAACAGCGCAACACGCTGGAGCAGCTGTTTAAAACCCCCGTGCCGCAGGG
TATTAAATGGGCCGACATTGAATCGCTAATTAAAGCGTTAGGTGGGGAGATTAAAGAAGGACGCGGCTCGCGCTGTAAAT
TTTTGTTGAACCACAGCATTGCCAGCTTTCACAGGCCTCACCCTTCGCCGGATACTGACAAAGGCGCCGTGGAGAGCGTG
CGCGATTGGTTAATCACTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8881.86 Da
• Isoelectric Point: 4.5702

>AT202011 WP_077983309.1 NZ_CP074393:c349034-348807 [Agrobacterium salinitolerans]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRIQNGESKVISAEEFWRDLDN

Nucleotide

• Length: 228 bp

>AT202011 NZ_CP099260:264553-264912 [Klebsiella aerogenes]
ATGATCAAACCTAAAACGCCAAATTCAATGGAGATTGCCGGGCAGCCTGCTGTTATTAACTACGTGCCTGAACTCAATGC
ATTCCGCGGTAAGTTCCTGGGATTATCAGGATATTGCGATTTTGTTTCCGATAGCATTCAAGGGTTACAACAGGAGGGCG
AGATATCATTACAGGAATATCTTGCTGATTGTCGTGAAGCGGGCATCGAGCCTTATGCTCAGCCGGAAAAGCTGAAAACC
TTCACATTGCGCTATCCTGAATCATTTGGTGAGCGTCTTAGCTCCGCCGCTGCAGAGGAGCAGGTTTCCGTAAATACCTG
GATCCTTGAGACGCTCAATGAACGTCTCAAACAGGCTTAA