Detailed information of TA system TA202009

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1316959..1317876
• Replicon: chromosome
• Accession: NZ_CP074391
• Organism: Bacillus amyloliquefaciens strain L-17

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: KHA81_RS06935
• Protein ID: WP_003154806.1
• Coordinates: 1317130..1317876 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KHA81_RS06930
• Protein ID: WP_003154807.1
• Coordinates: 1316959..1317129 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHA81_RS06895	1312190..1313812	+	1623	WP_014304855.1	pyocin knob domain-containing protein	-
KHA81_RS06900	1313825..1314196	+	372	WP_014304856.1	XkdW family protein	-
KHA81_RS06905	1314202..1314399	+	198	WP_003154819.1	XkdX family protein	-
KHA81_RS06910	1314456..1315217	+	762	WP_014304857.1	hypothetical protein	-
KHA81_RS06915	1315269..1315532	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KHA81_RS06920	1315546..1315809	+	264	WP_003154813.1	phage holin	-
KHA81_RS06925	1315823..1316701	+	879	WP_014304858.1	N-acetylmuramoyl-L-alanine amidase	-
KHA81_RS19585	1316737..1316862	-	126	WP_003154809.1	hypothetical protein	-
KHA81_RS06930	1316959..1317129	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KHA81_RS06935	1317130..1317876	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KHA81_RS06940	1317981..1318979	-	999	WP_003154805.1	inorganic phosphate transporter	-
KHA81_RS06945	1318992..1319609	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KHA81_RS06950	1319895..1321211	-	1317	WP_003154801.1	amino acid permease	-
KHA81_RS06955	1321535..1322485	+	951	WP_015388352.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1270136..1375297	105161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T202009 WP_003154806.1 NZ_CP074391:c1317876-1317130 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T202009 NZ_CP099260:c53694-53380 [Klebsiella aerogenes]
GTGCAATATTATGTCTACAGAAGTACCAGTAAAAACCCGGCCTTCCCACTTTTAATTGATGTTACCAGTGACATTATTGG
CGACATTAATCGCCGCTTAGCGATCCCGTTAATGCCCGTTGAGCGACTCGGTGTTAATCCCGCGCCGGAGCGTTTAAACC
CGATACTGGCTTTGGTTGACGGGAAAAAATACGTTTTGATGACACACGAAGCGGCAACCATTCCTGTTAAAGCCTTAGGA
GTCGAGTTTTGCGATGCGAGCGGATACCGCAACGCTATTAAAGGAGCGCTCGATTTCATGCTTGATGGCATTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT202009 WP_003154807.1 NZ_CP074391:c1317129-1316959 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT202009 NZ_CP099260:c53930-53697 [Klebsiella aerogenes]
ATGACTGTAAAACGTAAAACGCAAAACGTGACCGTCACTCTCGAACGGGATTTACTCAATCGTGCGCGCGAAGCCGGTGT
AAATATGAGCGCGACATTAGCCGCAGCGCTTGATGCTGAGCTGAAAAAACATGCTGCCCTGCGTTGGCGAGAAGCTAATG
CTGAAGCAATAGCTGCAATGAATGCTTTAGGGGAAGAGACCGGATGCTTTTCTGATGAATATCGGAGTTTTTGA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14