Detailed information of TA system TA202008

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 523574..524211
• Replicon: chromosome
• Accession: NZ_CP074391
• Organism: Bacillus amyloliquefaciens strain L-17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KHA81_RS02680
• Protein ID: WP_003156187.1
• Coordinates: 523861..524211 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KHA81_RS02675
• Protein ID: WP_003156188.1
• Coordinates: 523574..523855 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHA81_RS02655	519939..520538	-	600	WP_015388676.1	rhomboid family intramembrane serine protease	-
KHA81_RS02660	520631..520996	+	366	WP_014304402.1	holo-ACP synthase	-
KHA81_RS02665	521161..522168	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
KHA81_RS02670	522285..523454	+	1170	WP_015388675.1	alanine racemase	-
KHA81_RS02675	523574..523855	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KHA81_RS02680	523861..524211	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KHA81_RS02685	524329..525150	+	822	WP_003156182.1	STAS domain-containing protein	-
KHA81_RS02690	525155..525520	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KHA81_RS02695	525523..525924	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KHA81_RS02700	525936..526943	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
KHA81_RS02705	527007..527336	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KHA81_RS02710	527333..527815	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
KHA81_RS02715	527781..528569	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KHA81_RS02720	528569..529171	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T202008 WP_003156187.1 NZ_CP074391:523861-524211 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T202008 NZ_CP099254:c4704246-4703935 [Citrobacter freundii]
ATGTTATTCATAGAAACGGAAATCTTTACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGACTACAGAT
ATTCCTTACGATTCAGCCCGATTGTGGCGATCTGATACAAGATACAGGGGGCCTGAGAAAGGTTCGCTGGAGAGCGAGAG
GGAAAGGAAAACGTGGCGGTGTAAGGATTATCTACTTTCATCAAATCAGGAAATCACAAATTCGTTTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAAGAAAAAGTACTGCTGCGTATGTTGAATGAGGGGTGGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT202008 WP_003156188.1 NZ_CP074391:523574-523855 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT202008 NZ_CP099254:c4703934-4703647 [Citrobacter freundii]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGATTCAGATGAATGAGATCGCTGAAGGAACGCGGCAGCCTTC
TCGTACCTTTCATATTGACGCCATGAAAATAAAGGAGATCCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTACGAAATTGGGAGCAGGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCCATTGCCAATGATCCAAAACATGTCATTCAGGCGCTAAGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5