Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2607711..2608362 Replicon chromosome
Accession NZ_CP074379
Organism Lacticaseibacillus zeae strain FBL8

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag KG087_RS12310 Protein ID WP_005686631.1
Coordinates 2607711..2608094 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R1EMB2
Locus tag KG087_RS12315 Protein ID WP_010491246.1
Coordinates 2608114..2608362 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG087_RS12290 (KG087_12290) 2603055..2604062 + 1008 WP_003662706.1 L-lactate dehydrogenase -
KG087_RS12295 (KG087_12295) 2604370..2605740 + 1371 WP_075761421.1 L,D-transpeptidase family protein -
KG087_RS12300 (KG087_12300) 2605955..2606620 + 666 WP_010491258.1 cyclic di-AMP binding protein CbpA -
KG087_RS12305 (KG087_12305) 2606943..2607641 + 699 WP_010491256.1 L,D-transpeptidase -
KG087_RS12310 (KG087_12310) 2607711..2608094 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KG087_RS12315 (KG087_12315) 2608114..2608362 - 249 WP_010491246.1 antitoxin Antitoxin
KG087_RS12320 (KG087_12320) 2608461..2609600 - 1140 WP_213449874.1 alanine racemase -
KG087_RS12325 (KG087_12325) 2609587..2609961 - 375 WP_010491243.1 holo-ACP synthase -
KG087_RS12330 (KG087_12330) 2610199..2611707 - 1509 WP_047107145.1 DEAD/DEAH box helicase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2598256..2709391 111135


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T202006 WP_005686631.1 NZ_CP074379:c2608094-2607711 [Lacticaseibacillus zeae]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T202006 NZ_CP099254:c4175290-4175003 [Citrobacter freundii]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAGTAACCACCGTAAACATGGTGTTCGCTTTGAGGACGCGGT
ACTCGTTTTCGACGACCCCCGGCATTTGTCCAGACAGGATCGTTATGAGAACGGTGAGTACCGGTGGCAGACCATCGGTC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGT
GCCAGAAAGGCAGATCGTAAAGAGAGGAATCGTTATGAGCATGATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8971.07 Da        Isoelectric Point: 4.6061

>AT202006 WP_010491246.1 NZ_CP074379:c2608362-2608114 [Lacticaseibacillus zeae]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT202006 NZ_CP099254:c4175016-4174714 [Citrobacter freundii]
ATGAGCATGATTAAACATAAACGTGGCAGCGCATCCGCGCTGAGTGCTCAGCATGAGGCGGAACTGAAGGCGCTGGCGAA
TAAACCGGATGATGATATTGATTATAGCGACATTCCGGTGACTGAAGATGAGCAGTGGTCAGAGGCTAATCGCGGTACGT
TTTTCCGTCCGTTGAAAACTCAGGCATCGGTGCGCATTGATGCCGATGTGATGGAGTGGTTAAAGCGCCCCGGGAAGGGT
TACCAGACGCGGCTAAATGCTATTTTGCGCGAGGCTATGCTGAGAGAGCAAAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A0R1EMB2

References