Detailed information of TA system TA202005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2422809..2423460
• Replicon: chromosome
• Accession: NZ_CP074378
• Organism: Lacticaseibacillus chiayiensis strain FBL7

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KG086_RS11530
• Protein ID: WP_129301156.1
• Coordinates: 2422809..2423192 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A0R1EMB2
• Locus tag: KG086_RS11535
• Protein ID: WP_010491246.1
• Coordinates: 2423212..2423460 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG086_RS11505 (KG086_11505)	2418333..2419583	-	1251	WP_213224100.1	RNA-guided endonuclease TnpB family protein	-
KG086_RS11510 (KG086_11510)	2419658..2420107	+	450	WP_129302982.1	IS200/IS605 family transposase	-
KG086_RS11515 (KG086_11515)	2420351..2421049	+	699	WP_129302926.1	L,D-transpeptidase	-
KG086_RS11520 (KG086_11520)	2421124..2422017	-	894	WP_129302966.1	IS3 family transposase	-
KG086_RS11525 (KG086_11525)	2422014..2422691	-	678	WP_129302967.1	helix-turn-helix domain-containing protein	-
KG086_RS11530 (KG086_11530)	2422809..2423192	-	384	WP_129301156.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KG086_RS11535 (KG086_11535)	2423212..2423460	-	249	WP_010491246.1	antitoxin	Antitoxin
KG086_RS11540 (KG086_11540)	2423559..2424698	-	1140	WP_129301157.1	alanine racemase	-
KG086_RS11545 (KG086_11545)	2424685..2425059	-	375	WP_049170363.1	holo-ACP synthase	-
KG086_RS11550 (KG086_11550)	2425301..2426812	-	1512	WP_129301158.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13784.06 Da
• Isoelectric Point: 10.9764

>T202005 WP_129301156.1 NZ_CP074378:c2423192-2422809 [Lacticaseibacillus chiayiensis]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVNLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T202005 NZ_CP099254:3650524-3650742 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8971.07 Da
• Isoelectric Point: 4.6061

>AT202005 WP_010491246.1 NZ_CP074378:c2423460-2423212 [Lacticaseibacillus chiayiensis]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT202005 NZ_CP099254:3650122-3650496 [Citrobacter freundii]
ATGGACGAGTACTCACCCAAACGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCGACTTCAGCAACCAGCCTGCAGCTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGAAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0R1EMB2