Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2469080..2469731 Replicon chromosome
Accession NZ_CP074377
Organism Lacticaseibacillus casei strain FBL6

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag KGS74_RS11645 Protein ID WP_005686631.1
Coordinates 2469348..2469731 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R1EMB2
Locus tag KGS74_RS11640 Protein ID WP_010491246.1
Coordinates 2469080..2469328 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KGS74_RS11625 (KGS74_11625) 2465723..2467234 + 1512 WP_049170360.1 DEAD/DEAH box helicase -
KGS74_RS11630 (KGS74_11630) 2467481..2467855 + 375 WP_049170363.1 holo-ACP synthase -
KGS74_RS11635 (KGS74_11635) 2467842..2468981 + 1140 WP_049170365.1 alanine racemase -
KGS74_RS11640 (KGS74_11640) 2469080..2469328 + 249 WP_010491246.1 antitoxin Antitoxin
KGS74_RS11645 (KGS74_11645) 2469348..2469731 + 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KGS74_RS11650 (KGS74_11650) 2469801..2470499 - 699 WP_049170367.1 L,D-transpeptidase -
KGS74_RS11655 (KGS74_11655) 2470695..2471360 - 666 WP_010491258.1 cyclic di-AMP binding protein CbpA -
KGS74_RS11660 (KGS74_11660) 2471568..2472938 - 1371 WP_049170368.1 L,D-transpeptidase family protein -
KGS74_RS11665 (KGS74_11665) 2473246..2474253 - 1008 WP_003662706.1 L-lactate dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T202004 WP_005686631.1 NZ_CP074377:2469348-2469731 [Lacticaseibacillus casei]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T202004 NZ_CP099254:2462621-2463103 [Citrobacter freundii]
ATGGAAATAAACGTCACGGCGCCGGCGTTGCTGACCGAAGGGCATGAGCTCCAGCCATTCGATTGCGGTAATGAGGTGTT
AAATGATTGGCTATGTCGCCGGGCGATTAAAAATCAGTACATCAACGCATCGCGCACATTCGTCATTTGTCTGGAGTCTA
CTCAGCGCGTAGTGGGTTACTATTCCATTGCAACTGGATCCGTGAGCCACGCTGACTTAGGGCGTAGCTTACGTCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTCTGTTGGGCCGTCTTGCTGTTGATGCTAGTACGCAAGGTCATAGTTTCGGAAAATG
GCTGCTTAATGACGCTGTACTGCGCATTTCTAACTTAGCCGATCAAGTGGGTATCAAAGCCATCATGGTACATGCTATTG
ATGGCAAGGCCAGAGCGTTCTATGAATATTATGGTTTCGTTCAGTCCCCAATAGCGGCAAATACGTTGTTCTACAAAATT
TAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8971.07 Da        Isoelectric Point: 4.6061

>AT202004 WP_010491246.1 NZ_CP074377:2469080-2469328 [Lacticaseibacillus casei]
MKKQTSVTVTFDAAIKARLDAYCELNDTDTQDVITTAVAKFLQSQAPDINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT202004 NZ_CP099254:2462334-2462630 [Citrobacter freundii]
CTGGAGAAACTCATGCAAACCAGCGCTCGTAAGCCAACTCGCGATAAACAAATTAATATCCGCGCGACTGATGAGGAACG
CGCAGTGATTGATTATGCCGCAAGTTTGGTTAATAAAAACCGCACTGACTTTATCATTGAGAAGGCCGTGCATGAGGCCC
AGAATATCATTCTGGATCAGCGTGTGTTCGTCCTGGACGATGCCCGATATCAGTCTTTCATTGAGCAGCTTGAAGCACCT
GTACAAAATGTTGAGGGGCGTCAACGCCTGATGGATGTGAAGCCAGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB A0A0R1EMB2

References