Detailed information of TA system TA202003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Txe-yefM/YoeB-RelB
• Location: 2358003..2358528
• Replicon: chromosome
• Accession: NZ_CP074377
• Organism: Lacticaseibacillus casei strain FBL6

Toxin (Protein)

• Gene name: Txe
• Uniprot ID: -
• Locus tag: KGS74_RS11050
• Protein ID: WP_191978409.1
• Coordinates: 2358310..2358528 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: KGS74_RS11045
• Protein ID: WP_049170985.1
• Coordinates: 2358003..2358278 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KGS74_RS11030 (KGS74_11030)	2353948..2354365	-	418	Protein_2142	MarR family transcriptional regulator	-
KGS74_RS11035 (KGS74_11035)	2354502..2355146	+	645	WP_049170980.1	HD domain-containing protein	-
KGS74_RS11040 (KGS74_11040)	2356235..2357836	+	1602	WP_049170983.1	ABC transporter ATP-binding protein	-
KGS74_RS11045 (KGS74_11045)	2358003..2358278	+	276	WP_049170985.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KGS74_RS11050 (KGS74_11050)	2358310..2358528	+	219	WP_191978409.1	Txe/YoeB family addiction module toxin	Toxin
KGS74_RS11060 (KGS74_11060)	2358976..2359623	+	648	WP_049170987.1	type II CAAX endopeptidase family protein	-
KGS74_RS11065 (KGS74_11065)	2359781..2360752	+	972	WP_049170989.1	TDT family transporter	-
KGS74_RS11070 (KGS74_11070)	2360777..2361601	-	825	WP_049170991.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-72)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8486.74 Da
• Isoelectric Point: 9.0700

>T202003 WP_191978409.1 NZ_CP074377:2358310-2358528 [Lacticaseibacillus casei]
ISWFEQADKATLRKINRLITSIDRTPFDSLGKPEPLKHGLSGMWSRRITQEHRLIYEIEANTMTIISCRDHY

Nucleotide

• Length: 219 bp

>T202003 NZ_CP099254:c2420529-2420251 [Citrobacter freundii]
ATGATCATGAACTTCAGACACAAAGGATTGCGCGACTTGTTTCTTCAGGGGCGAACATCAGGCGTGACGGCGAAGCATGT
CCGGAGATTGCGGCACCGACTTGCCGTTATTGATGCAGCGAGCCATGTAACAGATATCAATATGCCTGGTTATAAACTGC
ATGCGTTAACGGGCGATCGCGACGGCGTTTGGTCGATTTCTGTTTCAGGCAACTGGCGTATTACGTTTGAATTCGTCAAT
GGCGATGCATATATACTGGATTACGAGGACTATCATTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10223.54 Da
• Isoelectric Point: 7.2022

>AT202003 WP_049170985.1 NZ_CP074377:2358003-2358278 [Lacticaseibacillus casei]
MDAVSYSVFRRNLKHYLQAVNDTSTPLLITSRDNEADTAVLMNKRDFDAMQETMRILSNPELMAKIRRGQAQIAAGKTRQ
HDLKDVGGGDD

Nucleotide

• Length: 276 bp

>AT202003 NZ_CP099254:c2420251-2419967 [Citrobacter freundii]
ATGAAAATGGCTAATCATCCCCGTCCGGGGGATATTATTCAGGAAGCGCTGGATGAGTTAAACGTCAGCCTGCGCGAATT
TGCCAGAGCAATGGAGATTGCCCCCTCAACCGCGAGCCGACTGTTAACGGGCAAGGCAGCATTAACCCCGGAAATGGCGA
TCAAACTTTCAGTGGTGATCGGCAGTTCGCCCGAAATGTGGTTGAATCTGCAGAATGCCTGGAGTCTGGCTGAAGCGCAA
AAAAGCGTGGATATCTCACGTCTGCGCAAACTGGTAGCACAGTAA