Detailed information of TA system TA202002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4481889..4482547
• Replicon: chromosome
• Accession: NZ_CP074376
• Organism: Mycolicibacterium neoaurum strain MN2019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MN2019_RS21290
• Protein ID: WP_213447571.1
• Coordinates: 4481889..4482287 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MN2019_RS21295
• Protein ID: WP_213447572.1
• Coordinates: 4482284..4482547 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN2019_RS21265 (MN2019_21265)	4477102..4477332	+	231	WP_213447567.1	antitoxin	-
MN2019_RS21270 (MN2019_21270)	4477319..4477662	+	344	Protein_4221	type II toxin-antitoxin system PemK/MazF family toxin	-
MN2019_RS21275 (MN2019_21275)	4477712..4478704	+	993	WP_213447568.1	ERCC4 domain-containing protein	-
MN2019_RS21280 (MN2019_21280)	4478701..4479993	+	1293	WP_213447569.1	DUF2786 domain-containing protein	-
MN2019_RS21285 (MN2019_21285)	4480187..4481848	-	1662	WP_213447570.1	SWIM zinc finger family protein	-
MN2019_RS21290 (MN2019_21290)	4481889..4482287	-	399	WP_213447571.1	type II toxin-antitoxin system VapC family toxin	Toxin
MN2019_RS21295 (MN2019_21295)	4482284..4482547	-	264	WP_213447572.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MN2019_RS21300 (MN2019_21300)	4482664..4483758	-	1095	WP_213447573.1	acyl-CoA desaturase	-
MN2019_RS21305 (MN2019_21305)	4483838..4484974	-	1137	Protein_4228	IS3 family transposase	-
MN2019_RS21310 (MN2019_21310)	4485281..4486063	-	783	WP_235733207.1	hypothetical protein	-
MN2019_RS21315 (MN2019_21315)	4486051..4487358	-	1308	WP_235733208.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4462617..4503464	40847

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-86)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14258.13 Da
• Isoelectric Point: 4.4331

>T202002 WP_213447571.1 NZ_CP074376:c4482287-4481889 [Mycolicibacterium neoaurum]
VIVDTSAIIAIALDEPERDALIEALLDAARPRMSAATSVEIAVLMRRLTPEDYRRVERLIERLGIESVPFDGEQAASAVR
AYRDFGRGSGHRAGLNLGDCYAYGLADVTGEPLLFVGDDFGHTDVAVAVRLS

Nucleotide

• Length: 399 bp

>T202002 NZ_CP099254:2031848-2031951 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9875.09 Da
• Isoelectric Point: 5.9111

>AT202002 WP_213447572.1 NZ_CP074376:c4482547-4482284 [Mycolicibacterium neoaurum]
MSLNIKNARTVELVRELARRTGLSQTGAVEEAVRAKLAEVQAGEGREARRTRIDVLLEELDQSVTAEQKKQIRDDERHMY
DDRGVPT

Nucleotide

• Length: 264 bp

>AT202002 NZ_CP099254:c2031957-2031722 [Citrobacter freundii]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGTCCGCGACCAAAGTGG
CCTGAAAAAAAGGACGATTGTCACCCGCTAGAATGCAAAAACCGCAAGTTCTGCAGCCAGAACGTTGCGGTTTTTT