Detailed information of TA system TA201999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1157856..1158895
• Replicon: chromosome
• Accession: NZ_CP074375
• Organism: Achromobacter deleyi strain As-55

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HLG70_RS05310
• Protein ID: WP_171663605.1
• Coordinates: 1158320..1158895 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: HLG70_RS05305
• Protein ID: WP_171663606.1
• Coordinates: 1157856..1158323 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLG70_RS05290 (HLG70_05290)	1153633..1154973	-	1341	WP_171663609.1	MFS transporter	-
HLG70_RS05295 (HLG70_05295)	1155048..1156709	-	1662	WP_171663608.1	choline dehydrogenase	-
HLG70_RS05300 (HLG70_05300)	1156841..1157698	+	858	WP_171663607.1	IclR family transcriptional regulator	-
HLG70_RS05305 (HLG70_05305)	1157856..1158323	+	468	WP_171663606.1	helix-turn-helix domain-containing protein	Antitoxin
HLG70_RS05310 (HLG70_05310)	1158320..1158895	+	576	WP_171663605.1	PIN domain-containing protein	Toxin
HLG70_RS05315 (HLG70_05315)	1158885..1159970	-	1086	WP_171663604.1	methyltransferase	-
HLG70_RS05320 (HLG70_05320)	1160127..1161047	+	921	WP_171663603.1	AraC family transcriptional regulator	-
HLG70_RS05325 (HLG70_05325)	1161209..1163344	+	2136	WP_234103404.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21354.30 Da
• Isoelectric Point: 5.9902

>T201999 WP_171663605.1 NZ_CP074375:1158320-1158895 [Achromobacter deleyi]
VRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSPQIHDEWKRNLLSNRGDLTSEQLDRTSALMDRAIPDGLVTGH
ETLIAGLSLPDPDDRHVLAAAIRCSASVIVTFNQKDFPAAALDPYGIEAQHPDEFVENLFDLDQAAVVSAAQRQRAQLKN
PTMDVNRYLDVLLRQGLVQTVKALSTYQAIL

Nucleotide

• Length: 576 bp

>T201999 NZ_CP099254:309889-310257 [Citrobacter freundii]
ATGACACTACAAAATATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTTACAGG
CATGCCTGATCCCGGTCGTACGGAAGCGCTGATGTACCGGGTATTAAATCAAATTGAATATGAAGGTGTGACCGATGTGT
GGTTGCCGGCAGCCATGCATTTGCTGACCATTTCTCGCGGGCACATTTTTAATGATGGTAACAAACGGACCGCGTTGTTT
ATTACTTTGCTGTTTTTAAAGCGTAATGGGATCGCGCTGTCTGCAAACCCCGATTTTGTTGCCATGACGGTTGAAGCCGC
AGCGGGACGACTCTCGTTAGAACAGATTGCACATCGTCTGCGACAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17384.86 Da
• Isoelectric Point: 5.2709

>AT201999 WP_171663606.1 NZ_CP074375:1157856-1158323 [Achromobacter deleyi]
MPHTALSKMVLPVEREVEAAVQGQRALAAYLATRFETQSIQILDDQNQAHNVELPTSALRLLVDILAELADGNAVQVVPI
HAELTTQEAADLLNVSRPHIVKLLEEGLLPFHRTGKHRRVRFADLMQFKAERERASEQAMEELARQAQELAMGYE

Nucleotide

• Length: 468 bp

>AT201999 NZ_CP099254:309671-309892 [Citrobacter freundii]
ATGCGTACAGTTAACTATAGTGAAGCGAGACAGAATCTGGCTGAAGTGCTGGATAGTGCGGTTACCGGAGTCCCCGTCAC
CATTACGCGACGCGGTCATAAACCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGTTATCAGGCCGCCAGGCTGGATG
ATGAATTTGCTGACATTATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA