Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 76781..77382 Replicon plasmid unnamed3
Accession NZ_CP074369
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag KG092_RS25375 Protein ID WP_001216034.1
Coordinates 77002..77382 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KG092_RS25370 Protein ID WP_001190712.1
Coordinates 76781..77002 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS25335 (KG092_25095) 71958..72136 + 179 Protein_73 hypothetical protein -
KG092_RS25340 72470..72604 + 135 Protein_74 hypothetical protein -
KG092_RS25345 72815..72922 + 108 Protein_75 hypothetical protein -
KG092_RS25350 (KG092_25105) 73765..74127 + 363 WP_009447741.1 hypothetical protein -
KG092_RS25355 (KG092_25110) 75203..75559 - 357 WP_032272825.1 hypothetical protein -
KG092_RS25360 (KG092_25115) 75560..76144 - 585 WP_032272826.1 hypothetical protein -
KG092_RS25365 (KG092_25120) 76319..76708 + 390 WP_000506730.1 S24 family peptidase -
KG092_RS25370 (KG092_25125) 76781..77002 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KG092_RS25375 (KG092_25130) 77002..77382 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KG092_RS25380 (KG092_25135) 77387..77566 + 180 WP_009446826.1 hypothetical protein -
KG092_RS25385 (KG092_25140) 77594..78637 + 1044 WP_009446825.1 DUF968 domain-containing protein -
KG092_RS25390 (KG092_25145) 78726..79178 + 453 WP_001326849.1 late promoter-activating protein -
KG092_RS25395 (KG092_25150) 79264..80457 + 1194 WP_032153803.1 hypothetical protein -
KG092_RS25400 (KG092_25155) 80499..81941 + 1443 WP_001472843.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..93492 93492


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T201996 WP_001216034.1 NZ_CP074369:77002-77382 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T201996 NZ_CP099252:c4699083-4698799 [Klebsiella aerogenes]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATGGAGTCGGCCAGGTTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCGTCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT201996 WP_001190712.1 NZ_CP074369:76781-77002 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT201996 NZ_CP099252:c4699315-4699073 [Klebsiella aerogenes]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCAGCGTTGTTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References