Detailed information of TA system TA201995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 74398..74902
• Replicon: plasmid unnamed2
• Accession: NZ_CP074368
• Organism: Escherichia coli strain ZYB62

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KG092_RS24900
• Protein ID: WP_275886520.1
• Coordinates: 74398..74682 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: KG092_RS24905
• Protein ID: WP_000813639.1
• Coordinates: 74684..74902 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG092_RS24885 (70499)	70499..71470	-	972	WP_000817031.1	ParB/RepB/Spo0J family plasmid partition protein	-
KG092_RS24890 (71470)	71470..72636	-	1167	WP_000772446.1	plasmid-partitioning protein SopA	-
KG092_RS24895 (73660)	73660..74364	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
KG092_RS24900 (74398)	74398..74682	-	285	WP_275886520.1	type II toxin-antitoxin system toxin CcdB	Toxin
KG092_RS24905 (74684)	74684..74902	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KG092_RS24910 (77007)	77007..77972	+	966	Protein_68	IS3 family transposase	-
KG092_RS24915 (78655)	78655..79491	+	837	WP_000222506.1	helix-turn-helix domain-containing protein	-
KG092_RS24920 (79519)	79519..79623	+	105	WP_001443026.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87829	87829
	-	flank	IS/Tn	-	-	73660..74364	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-89)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10831.41 Da
• Isoelectric Point: 5.6993

>T201995 WP_275886520.1 NZ_CP074368:c74682-74398 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENEALLQS

Nucleotide

• Length: 285 bp

>T201995 NZ_CP099252:c4619151-4618777 [Klebsiella aerogenes]
ATGCAAATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACAACACACCGTTTTGCAACGACAGTGTGATCCAGAAGCACATTG
ATGCGGGTATCTCACTGTGTGACGCGGTGAATTTTATCGTGGAAAAGTACGAGCTGGTACGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGCAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT201995 WP_000813639.1 NZ_CP074368:c74902-74684 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT201995 NZ_CP099252:c4619560-4619201 [Klebsiella aerogenes]
ATGCAGAAAGCAACCTGTGTAATCAACCATAATATCGCGGAACCCTGGTGGGGCCTCAGACGTAACATCACGCCGTGTTT
TGGTGCCCGGCTGGTACAGGAGGGTAACCACCTACATTACCTTGCTGACCGCGCCAGCATTGCTGGGACATTCAGCGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGACCAGCAACGAACTCACG
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATCT
GTACATCGTAATTTATCCGACACCTGCAACAACCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DIR5