Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 40050..40693 | Replicon | plasmid unnamed2 |
| Accession | NZ_CP074368 | ||
| Organism | Escherichia coli strain ZYB62 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | B1LRW4 |
| Locus tag | KG092_RS24730 | Protein ID | WP_001044768.1 |
| Coordinates | 40277..40693 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D2WFK3 |
| Locus tag | KG092_RS24725 | Protein ID | WP_001261287.1 |
| Coordinates | 40050..40280 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KG092_RS24700 (35516) | 35516..35866 | - | 351 | WP_000624722.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| KG092_RS24705 (35863) | 35863..36288 | - | 426 | WP_000422741.1 | transposase | - |
| KG092_RS24710 (36342) | 36342..38525 | + | 2184 | WP_275886521.1 | P-loop NTPase fold protein | - |
| KG092_RS24715 (38575) | 38575..39474 | - | 900 | WP_000963206.1 | nucleotide-binding protein | - |
| KG092_RS24720 (39464) | 39464..39754 | - | 291 | WP_000111771.1 | hypothetical protein | - |
| KG092_RS24725 (40050) | 40050..40280 | + | 231 | WP_001261287.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| KG092_RS24730 (40277) | 40277..40693 | + | 417 | WP_001044768.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KG092_RS24735 (40855) | 40855..42993 | - | 2139 | WP_000350638.1 | AAA family ATPase | - |
| KG092_RS24740 (43347) | 43347..43604 | + | 258 | WP_000371891.1 | hypothetical protein | - |
| KG092_RS24745 (43604) | 43604..44194 | + | 591 | WP_198447382.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..87829 | 87829 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15197.66 Da Isoelectric Point: 7.7805
>T201990 WP_001044768.1 NZ_CP074368:40277-40693 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK
Download Length: 417 bp
>T201990 NZ_CP099252:1906746-1906849 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT
Antitoxin
Download Length: 77 a.a. Molecular weight: 8815.90 Da Isoelectric Point: 4.3775
>AT201990 WP_001261287.1 NZ_CP074368:40050-40280 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT201990 NZ_CP099252:c1906857-1906711 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A606Q844 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9QFC4 |