Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 193263..194886 Replicon plasmid unnamed1
Accession NZ_CP074367
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KG092_RS24380 Protein ID WP_000088046.1
Coordinates 193263..194588 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KG092_RS24385 Protein ID WP_024136327.1
Coordinates 194593..194886 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS24350 (KG092_24110) 189528..189908 + 381 WP_001426317.1 hypothetical protein -
KG092_RS24355 (KG092_24115) 189966..190631 + 666 WP_000490638.1 hypothetical protein -
KG092_RS24365 (KG092_24125) 191656..192471 - 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KG092_RS24370 (KG092_24130) 192484..192894 - 411 WP_000985911.1 hypothetical protein -
KG092_RS24375 (KG092_24135) 192996..193202 - 207 WP_000134171.1 hypothetical protein -
KG092_RS24380 (KG092_24140) 193263..194588 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KG092_RS24385 (KG092_24145) 194593..194886 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KG092_RS24390 (KG092_24150) 195490..195882 - 393 WP_000341066.1 hypothetical protein -
KG092_RS24395 (KG092_24155) 196255..196596 + 342 WP_001151575.1 hypothetical protein -
KG092_RS24400 (KG092_24160) 196688..196879 + 192 WP_000398480.1 hypothetical protein -
KG092_RS24405 (KG092_24165) 196929..197210 + 282 WP_000077926.1 hypothetical protein -
KG092_RS24410 197551..197682 - 132 WP_000374059.1 hypothetical protein -
KG092_RS24415 (KG092_24170) 197773..199014 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / qacE / sul1 / aac(3)-IId / mph(A) / blaCTX-M-55 / floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B - 1..226158 226158


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T201989 WP_000088046.1 NZ_CP074367:c194588-193263 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T201989 NZ_CP099252:851476-851886 [Klebsiella aerogenes]
GTGGTCCTGTGGCAATCTGATTTACGTATTTCCTGGCGTTCGCAGTGGTTCTCGCTGCTGCTGCACGGGGTGGTGGCGGC
GATAGTGCTGCTCATGCCCTGGCCGTTAAGCTACACCCCGATTTGGCTCCTGTTGCTATCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCACGCCTGCCAGGGAGAAATTAAGCTGTTGATCGATTCCCGTTTACGGTGGCAAAAAACG
GAATGGGATATCGTCGGGACGCCATGGGTTATCAGCAGCGGTATGCTGCTGCGCCTGAAGCATGCGGAAACCGGCCGTAG
CCAGCATTTGTGGGTGGCGGCGGATAGCATGGATGCGGGGGAATGGCGGGATTTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCACGACTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT201989 WP_024136327.1 NZ_CP074367:c194886-194593 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT201989 NZ_CP099252:851229-851495 [Klebsiella aerogenes]
ATGGACATTAACAACAAAGCTCGCATCCACTGGGCATGCCGCCGCGGTATGCGCGAACTCGATATCTCCATCATGCCGTT
TTTCGAGTATGAGTACGACACGCTCAGCGATGACGACAAGCGCCTGTTCATCCGTTTGCTGGAGAACGATGATCCGGATC
TGTTTAACTGGCTGATGAATCACGGCAAGCCGGCAGATGCGCAACTTCAACGGATGATTAATTTAATCCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References