Detailed information of TA system TA201985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3598482..3599319
• Replicon: chromosome
• Accession: NZ_CP074366
• Organism: Escherichia coli strain ZYB62

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KG092_RS17430
• Protein ID: WP_000227784.1
• Coordinates: 3598777..3599319 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KG092_RS17425
• Protein ID: WP_001297137.1
• Coordinates: 3598482..3598793 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG092_RS17400 (3593502)	3593502..3594449	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KG092_RS17405 (3594471)	3594471..3596462	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KG092_RS17410 (3596452)	3596452..3597066	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KG092_RS17415 (3597066)	3597066..3597395	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KG092_RS17420 (3597407)	3597407..3598297	+	891	WP_000971336.1	heme o synthase	-
KG092_RS17425 (3598482)	3598482..3598793	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KG092_RS17430 (3598777)	3598777..3599319	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KG092_RS17435 (3599375)	3599375..3600310	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
KG092_RS17440 (3600718)	3600718..3602082	+	1365	WP_001000978.1	MFS transporter	-
KG092_RS17445 (3602210)	3602210..3602701	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KG092_RS17450 (3602869)	3602869..3603780	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T201985 WP_000227784.1 NZ_CP074366:3598777-3599319 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T201985 NZ_CP099222:4255041-4255328 [Citrobacter freundii]
ATGAGTTATACGGTAAAGTTCAGGGAAGATGCGCTGAAAGAATGGCAAAAACTGGATAAAACCATTCAGCAGCAGTTTGC
CAAAAAACTCAAGAAATGCGGTGAAAATCCGCATGTCCCCTCCGCGAAGCTCAGGGGAATAAAAGATTGCTATAAAATCC
AACTACGCACTTCAAGGTTCCGTCTTGTTTATCAAGTTATTGACGACACCCTGGTCATCGCTGTCGTTGCTGTCGGTAAA
CGCGAGCGTAGTGAAGTTTATAATCTTGCCAGCGAAAGACTACGTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT201985 WP_001297137.1 NZ_CP074366:3598482-3598793 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT201985 NZ_CP099222:4254793-4255044 [Citrobacter freundii]
ATGCCACATATCATTTTAAGCGACACCACCGCGAGCGTGAGTGAGCTAAAAAAGAACCCGATGGCGACTGTTAGCGCAGG
GGATGGGTTTCCGGTCGCAATCCTGAACCGTAACCAGCCCGCCTTTTATTGTGTTCCCGCTGAGCTTTACGAAAAAATGC
TTGATGCGCTGGACGATCAGCAACTGGTTAAATTAGTCCATGAACGGAACCAACAGCCACTGGTGGATGTTGATCTGGAC
AGCTATTTATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3