Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3564405..3565023 Replicon chromosome
Accession NZ_CP074366
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KG092_RS17260 Protein ID WP_001291435.1
Coordinates 3564805..3565023 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KG092_RS17255 Protein ID WP_000344800.1
Coordinates 3564405..3564779 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS17245 (3559494) 3559494..3560687 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KG092_RS17250 (3560710) 3560710..3563859 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KG092_RS17255 (3564405) 3564405..3564779 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KG092_RS17260 (3564805) 3564805..3565023 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KG092_RS17265 (3565195) 3565195..3565746 + 552 WP_000102568.1 maltose O-acetyltransferase -
KG092_RS17270 (3565862) 3565862..3566332 + 471 WP_000136192.1 YlaC family protein -
KG092_RS17275 (3566496) 3566496..3568046 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KG092_RS17280 (3568088) 3568088..3568441 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KG092_RS17290 (3568820) 3568820..3569131 + 312 WP_000409911.1 MGMT family protein -
KG092_RS17295 (3569162) 3569162..3569734 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T201984 WP_001291435.1 NZ_CP074366:3564805-3565023 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T201984 NZ_CP099222:c4082653-4082369 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACAGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTAGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT201984 WP_000344800.1 NZ_CP074366:3564405-3564779 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT201984 NZ_CP099222:c4082885-4082643 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGATAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCAGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGATA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References