Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 2915261..2916981 Replicon chromosome
Accession NZ_CP074366
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name pmenT Uniprot ID A0A7U9J2A7
Locus tag KG092_RS14230 Protein ID WP_000312833.1
Coordinates 2915261..2915917 (+) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag KG092_RS14235 Protein ID WP_000634203.1
Coordinates 2915914..2916981 (+) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS14215 (2911245) 2911245..2911421 + 177 WP_001056893.1 hypothetical protein -
KG092_RS14220 (2911461) 2911461..2913899 + 2439 WP_000985462.1 type I restriction-modification system subunit M -
KG092_RS14225 (2913899) 2913899..2915248 + 1350 WP_000228011.1 restriction endonuclease subunit S -
KG092_RS14230 (2915261) 2915261..2915917 + 657 WP_000312833.1 toxin-antitoxin system toxin DNA ADP-ribosyl transferase DarT Toxin
KG092_RS14235 (2915914) 2915914..2916981 + 1068 WP_000634203.1 toxin-antitoxin system antitoxin DNA ADP-ribosyl glycohydrolase DarG Antitoxin
KG092_RS14240 (2917000) 2917000..2920095 + 3096 WP_040090604.1 HsdR family type I site-specific deoxyribonuclease -
KG092_RS14245 (2920095) 2920095..2920811 + 717 WP_001122107.1 SprT family zinc-dependent metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - sfaX 2908853..2939795 30942


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25233.56 Da        Isoelectric Point: 6.2145

>T201983 WP_000312833.1 NZ_CP074366:2915261-2915917 [Escherichia coli]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLDSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRAGWYFS

Download         Length: 657 bp

>T201983 NZ_CP099222:3536864-3537082 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATCTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT201983 WP_000634203.1 NZ_CP074366:2915914-2916981 [Escherichia coli]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp

>AT201983 NZ_CP099222:3536462-3536836 [Citrobacter freundii]
ATGGACGAGTACTCACCCAAACGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCGACTTCAGCAACCAGCCTGCAGCTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGAAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9J2A7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References