Detailed information of TA system TA201980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2499816..2500454
• Replicon: chromosome
• Accession: NZ_CP074366
• Organism: Escherichia coli strain ZYB62

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KG092_RS12105
• Protein ID: WP_000813794.1
• Coordinates: 2500278..2500454 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KG092_RS12100
• Protein ID: WP_001270286.1
• Coordinates: 2499816..2500232 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KG092_RS12080 (2494968)	2494968..2495909	-	942	WP_001251319.1	ABC transporter permease	-
KG092_RS12085 (2495910)	2495910..2496923	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KG092_RS12090 (2496941)	2496941..2498086	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KG092_RS12095 (2498331)	2498331..2499737	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
KG092_RS12100 (2499816)	2499816..2500232	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KG092_RS12105 (2500278)	2500278..2500454	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KG092_RS12110 (2500676)	2500676..2500906	+	231	WP_000494244.1	YncJ family protein	-
KG092_RS12115 (2500998)	2500998..2502959	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KG092_RS12120 (2503032)	2503032..2503568	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
KG092_RS12125 (2503621)	2503621..2504835	+	1215	WP_001349372.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2504875..2506140	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T201980 WP_000813794.1 NZ_CP074366:c2500454-2500278 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T201980 NZ_CP074366:c2500454-2500278 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT201980 WP_001270286.1 NZ_CP074366:c2500232-2499816 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT201980 NZ_CP074366:c2500232-2499816 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB