Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1072272..1072855 Replicon chromosome
Accession NZ_CP074366
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KG092_RS05180 Protein ID WP_000254745.1
Coordinates 1072520..1072855 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KG092_RS05175 Protein ID WP_000581937.1
Coordinates 1072272..1072520 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS05165 (1068611) 1068611..1069912 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KG092_RS05170 (1069960) 1069960..1072194 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KG092_RS05175 (1072272) 1072272..1072520 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KG092_RS05180 (1072520) 1072520..1072855 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KG092_RS05185 (1072926) 1072926..1073717 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KG092_RS05190 (1073945) 1073945..1075582 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KG092_RS05195 (1075670) 1075670..1076968 + 1299 WP_000036723.1 phosphopyruvate hydratase -
KG092_RS05200 (1077024) 1077024..1077386 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T201967 WP_000254745.1 NZ_CP074366:1072520-1072855 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T201967 NZ_CP099217:928373-928759 [Citrobacter freundii]
ATGCAAACGAAATTACCCTTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTTGATGTCTGGCAGACTCTCCTGACCTG
CCTGCTGGAGCACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAACAGGTGATTCAGCAGCATATCGATG
CGGGTATCTCGCTGGTCGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGGCCAGGCTTCAGC
ATCATGGAGCAGTCTCCGTTTATTACGGCGATTGATATCCTCCGGGCCAGAAAGGCTACGGGGCTGATGAACCGTGGTAC
GTATAAAGAGGTGACGGCTATTACCAGAGGACAACATCCGCAGGCACGCACTTCAGGCAAGCGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT201967 WP_000581937.1 NZ_CP074366:1072272-1072520 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT201967 NZ_CP099217:927957-928316 [Citrobacter freundii]
TTGTCGAAGAACACCACGATAACAACACATGATATCAGCGAACCCTGGTGGGGACTCAGACGCAGTGTTTCCCCGTGTTT
TGGTGCCCGTCTGGTCCAGGAGGGGAACCGCCTGTATTACCTGGCTGACCGGGCGAATGTTAGCGGACAGTTCTGTGATG
CAGATTTGCGCCATCTTGACCAGTCTTTCCCGGTATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAAT
CCCCGTCATCAGCACGGCGTCACACTGTGTGCAAAAGGCCTGACCTGCGAGGCCGATACCCTCGGTTCGCATGGCTACGT
TTATATCGCGATTTATCCCACTCCCGTCACCACAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References