Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 273622..274208 Replicon chromosome
Accession NZ_CP074366
Organism Escherichia coli strain ZYB62

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag KG092_RS01220 Protein ID WP_042014162.1
Coordinates 273622..273852 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KG092_RS01225 Protein ID WP_000593555.1
Coordinates 273849..274208 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KG092_RS01210 (269659) 269659..272394 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
KG092_RS01215 (272394) 272394..273518 + 1125 WP_001314210.1 ABC transporter permease -
KG092_RS01220 (273622) 273622..273852 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
KG092_RS01225 (273849) 273849..274208 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KG092_RS01230 (274328) 274328..274729 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KG092_RS01235 (274735) 274735..275541 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
KG092_RS01240 (275538) 275538..276302 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
KG092_RS01245 (276302) 276302..277135 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KG092_RS01250 (277132) 277132..278076 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T201963 WP_042014162.1 NZ_CP074366:273622-273852 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T201963 NZ_CP099217:302188-302556 [Citrobacter freundii]
ATGACGCTACAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTGACGCCTGGTGTCGCAGG
TATGCCTGACCCCGGTCGTGCGGAAGCGCTGATGTATCGGGTATTGAATCAAATCGAATATGAAGGTGTGACTGACGTGT
GGCTACTGGCGGCAATGCATTTGTTAGCCATTTCCCGTGGGCATATCTTTAATGATGGTAACAAGCGTACTGCGTTATTT
ATTACGTTGCTGTTTTTAAAACGTAATGGGATCGTATTATCTGCAAACCCTGATTTTGTCGCCATGACGGTTGAAGCGGC
AGCAGGACAACTCACGTTAGAACAAATTGCGCATCGTCTGCGCCATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT201963 WP_000593555.1 NZ_CP074366:273849-274208 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT201963 NZ_CP099217:301970-302191 [Citrobacter freundii]
ATGCGCACTATGAACTATAGTGAAGCTCGACAAAACCTGGCTGAAGTGCTGGAGAGCGCTGTCACTGGTGTGCCTGTTAC
CATTACGCGCCGTGGTCATAAACCTGCGGTAATCATCAGCGCGGAAGAGTTTGAACGCTATCAGGCCGCCAGACTTGATG
ATGAATTTGCCGACATAATGATGATTCATGGTAATGAAATCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References