Detailed information of TA system TA201951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2582681..2583351
• Replicon: chromosome
• Accession: NZ_CP074363
• Organism: Xanthomonas translucens pv. secalis strain CFBP 2539

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KAF26_RS11065
• Protein ID: WP_003473425.1
• Coordinates: 2582681..2583100 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S7EM03
• Locus tag: KAF26_RS11070
• Protein ID: WP_003473422.1
• Coordinates: 2583097..2583351 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAF26_RS11025 (KAF26_10910)	2577903..2578271	-	369	WP_003466572.1	conjugal transfer transcriptional regulator TraJ	-
KAF26_RS11030 (KAF26_10915)	2578274..2578558	-	285	WP_003466574.1	hypothetical protein	-
KAF26_RS11035 (KAF26_10920)	2578570..2578812	-	243	WP_003466577.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
KAF26_RS11040 (KAF26_10925)	2578926..2580326	-	1401	WP_003466580.1	P-type conjugative transfer protein TrbL	-
KAF26_RS11045 (KAF26_10930)	2580328..2580486	-	159	WP_155522240.1	hypothetical protein	-
KAF26_RS11050 (KAF26_10935)	2580497..2581192	-	696	Protein_2184	P-type conjugative transfer protein TrbJ	-
KAF26_RS11055 (KAF26_10940)	2581300..2581593	+	294	WP_038237253.1	transposase	-
KAF26_RS11060 (KAF26_10945)	2581590..2582450	+	861	WP_060748765.1	IS3 family transposase	-
KAF26_RS11065 (KAF26_10950)	2582681..2583100	-	420	WP_003473425.1	type II toxin-antitoxin system VapC family toxin	Toxin
KAF26_RS11070 (KAF26_10955)	2583097..2583351	-	255	WP_003473422.1	hypothetical protein	Antitoxin
KAF26_RS11075 (KAF26_10960)	2583763..2584044	-	282	WP_003473419.1	TraK family protein	-
KAF26_RS11080 (KAF26_10965)	2584690..2585732	+	1043	WP_230938149.1	IS3 family transposase	-
KAF26_RS11085 (KAF26_10970)	2585850..2586331	+	482	Protein_2191	IS3 family transposase	-
KAF26_RS11090 (KAF26_10975)	2586433..2587583	+	1151	WP_199222609.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2582223..2587366	5143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14944.21 Da
• Isoelectric Point: 5.2082

>T201951 WP_003473425.1 NZ_CP074363:c2583100-2582681 [Xanthomonas translucens pv. secalis]
MIVLDTNVVSEAMKPEPNPAVRAWLNEQVAETLYLSSVTLAELLFGIGALPNGKRKKGLGEALDGLLELFSERVLMFDTE
AARNYAELAVKARIAGKGFPTPDGYIGAIAASKGFIVATRDTSPFEAAGLTVINPWNHR

Nucleotide

• Length: 420 bp

>T201951 NZ_CP099128:c4716454-4716080 [Citrobacter freundii]
ATGGTCAAAGGATCCGCGCTTTTTGATACCAACATCCTCATCGATCTGTTTAGTGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAATGCAATCAGCCTCATTACATGGATGGAGGTGATGGTGGGTGCAAAAAAATACCATCAGG
AAGCCCGTACTCGTGTTGCGCTAGGTGCTTTTAACGTGATCGGCGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGACAGGAATATGGGATGAAATTGCCGGATGCCATTATTTTGGCAACAGCACAGATTCATCGTCTTACGCTGGTTACGCG
AAATAATAAGGATTTTGCAGGCCTATCCGGTGTGGTCACACCGTATACATTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9507.88 Da
• Isoelectric Point: 7.2071

>AT201951 WP_003473422.1 NZ_CP074363:c2583351-2583097 [Xanthomonas translucens pv. secalis]
MAILTVRNVPDEVHRALRLRAAEHGRSTEAEVREILESAVKSQKRIRMGDALAELGRRVGLTNDDFAVLDQVRDKVPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT201951 NZ_CP099128:c4716696-4716454 [Citrobacter freundii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAAGTGATTAAACGTCTTGATGATCTCAAGGT
GCAGCGCAACCTGCCGCGAGCAGAACTTTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAGGATCAGGCTGAAACCA
CAATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A2S7EM03