Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 12911..13554 Replicon plasmid unnamed1
Accession NZ_CP074360
Organism Escherichia coli strain XJ6.2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KFQ22_RS22575 Protein ID WP_001044768.1
Coordinates 12911..13327 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KFQ22_RS22580 Protein ID WP_001261287.1
Coordinates 13324..13554 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ22_RS22560 (KFQ22_22270) 9410..10000 - 591 WP_000194575.1 hypothetical protein -
KFQ22_RS22565 (KFQ22_22275) 10000..10257 - 258 WP_000343085.1 hypothetical protein -
KFQ22_RS22570 (KFQ22_22280) 10611..12749 + 2139 WP_000350635.1 AAA family ATPase -
KFQ22_RS22575 (KFQ22_22285) 12911..13327 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KFQ22_RS22580 (KFQ22_22290) 13324..13554 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFQ22_RS22585 (KFQ22_22295) 13862..15427 + 1566 WP_000741348.1 AAA family ATPase -
KFQ22_RS22590 (KFQ22_22300) 15484..16353 - 870 WP_000253407.1 hypothetical protein -
KFQ22_RS22595 (KFQ22_22305) 16355..17443 - 1089 WP_000952231.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / sitABCD / blaCTX-M-102 / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / sul3 iroN / iroE / iroD / iroC / iroB 1..154679 154679


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T201947 WP_001044768.1 NZ_CP074360:c13327-12911 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T201947 NZ_CP099128:c2909030-2908698 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACTAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT201947 WP_001261287.1 NZ_CP074360:c13554-13324 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT201947 NZ_CP099128:c2909288-2909031 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References