201944

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3918695..3919271 Replicon chromosome
Accession NZ_CP074359
Organism Escherichia coli strain XJ6.2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFQ22_RS19050 Protein ID WP_001297643.1
Coordinates 3918984..3919271 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KFQ22_RS19045 Protein ID WP_000063156.1
Coordinates 3918695..3918997 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ22_RS19030 (3915291) 3915291..3917441 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFQ22_RS19035 (3917491) 3917491..3917694 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFQ22_RS19040 (3917705) 3917705..3918661 + 957 WP_001297640.1 GTPase -
KFQ22_RS19045 (3918695) 3918695..3918997 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KFQ22_RS19050 (3918984) 3918984..3919271 - 288 WP_001297643.1 BrnT family toxin Toxin
KFQ22_RS19055 (3919470) 3919470..3920167 - 698 Protein_3721 IS1 family transposase -
KFQ22_RS19060 (3920224) 3920224..3920319 + 96 Protein_3722 dienelactone hydrolase family protein -
KFQ22_RS19065 (3920465) 3920465..3921697 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
KFQ22_RS19070 (3921738) 3921738..3923018 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T201944 WP_001297643.1 NZ_CP074359:c3919271-3918984 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201944 NZ_CP099128:825755-826162 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT201944 WP_000063156.1 NZ_CP074359:c3918997-3918695 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201944 NZ_CP099128:825508-825774 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References