201929

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1766430..1767261 Replicon chromosome
Accession NZ_CP074359
Organism Escherichia coli strain XJ6.2

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KFQ22_RS08445 Protein ID WP_000854814.1
Coordinates 1766430..1766804 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag KFQ22_RS08450 Protein ID WP_001285585.1
Coordinates 1766893..1767261 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ22_RS08405 (1761826) 1761826..1762992 + 1167 WP_000830144.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
KFQ22_RS08410 (1763111) 1763111..1763584 + 474 WP_001105416.1 DNA gyrase inhibitor SbmC -
KFQ22_RS08415 (1763782) 1763782..1764840 + 1059 WP_001200890.1 FUSC family protein -
KFQ22_RS08420 (1765012) 1765012..1765341 + 330 WP_000450409.1 DUF496 family protein -
KFQ22_RS08425 (1765442) 1765442..1765576 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
KFQ22_RS08430 (1765696) 1765696..1765824 + 129 Protein_1645 transposase domain-containing protein -
KFQ22_RS08435 (1766113) 1766113..1766193 - 81 Protein_1646 hypothetical protein -
KFQ22_RS08440 (1766239) 1766239..1766433 - 195 WP_000988600.1 DUF5983 family protein -
KFQ22_RS08445 (1766430) 1766430..1766804 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KFQ22_RS08450 (1766893) 1766893..1767261 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KFQ22_RS08455 (1767335) 1767335..1767556 - 222 WP_000692323.1 DUF987 domain-containing protein -
KFQ22_RS08460 (1767619) 1767619..1768095 - 477 WP_001186773.1 RadC family protein -
KFQ22_RS08465 (1768111) 1768111..1768590 - 480 WP_000860081.1 antirestriction protein -
KFQ22_RS08470 (1768672) 1768672..1769493 - 822 WP_048217813.1 DUF932 domain-containing protein -
KFQ22_RS08475 (1769714) 1769714..1770124 - 411 WP_000846713.1 hypothetical protein -
KFQ22_RS08480 (1770140) 1770140..1770823 - 684 WP_000775500.1 hypothetical protein -
KFQ22_RS08485 (1770959) 1770959..1772029 - 1071 WP_000102644.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T201929 WP_000854814.1 NZ_CP074359:c1766804-1766430 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T201929 NZ_CP099084:599156-599440 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACAGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATTGACTCTGCCTGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAAGTTGTGATTGTGTTTGTTGTCGCGGTAGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT201929 WP_001285585.1 NZ_CP074359:c1767261-1766893 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT201929 NZ_CP099084:598924-599166 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGATAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGATA
GTGAAGATGCACTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGATTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References