Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 212732..214355 Replicon plasmid unnamed1
Accession NZ_CP074357
Organism Escherichia coli strain XJ6.1

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KFQ18_RS24335 Protein ID WP_000088046.1
Coordinates 212732..214057 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KFQ18_RS24340 Protein ID WP_024136327.1
Coordinates 214062..214355 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ18_RS24295 (KFQ18_24050) 207753..208109 + 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -
KFQ18_RS24300 (KFQ18_24055) 208451..208654 - 204 WP_001194555.1 hypothetical protein -
KFQ18_RS24305 (KFQ18_24060) 208716..209573 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KFQ18_RS24310 (KFQ18_24065) 209589..209897 - 309 WP_001224687.1 hypothetical protein -
KFQ18_RS24315 (KFQ18_24070) 210446..210871 - 426 WP_001043843.1 hypothetical protein -
KFQ18_RS24320 (KFQ18_24075) 211125..211940 - 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
KFQ18_RS24325 (KFQ18_24080) 211953..212363 - 411 WP_000985911.1 hypothetical protein -
KFQ18_RS24330 (KFQ18_24085) 212465..212671 - 207 WP_000134171.1 hypothetical protein -
KFQ18_RS24335 (KFQ18_24090) 212732..214057 - 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KFQ18_RS24340 (KFQ18_24095) 214062..214355 - 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KFQ18_RS24345 (KFQ18_24100) 214959..215351 - 393 WP_000341066.1 hypothetical protein -
KFQ18_RS24350 (KFQ18_24105) 215724..216065 + 342 WP_001151575.1 hypothetical protein -
KFQ18_RS24355 (KFQ18_24110) 216157..216348 + 192 WP_000398480.1 hypothetical protein -
KFQ18_RS24360 (KFQ18_24115) 216398..216679 + 282 WP_000077926.1 hypothetical protein -
KFQ18_RS24365 217020..217151 - 132 WP_000374059.1 hypothetical protein -
KFQ18_RS24370 (KFQ18_24120) 217242..218483 - 1242 WP_001053338.1 VWA domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA14 / ARR-3 / mph(A) / qnrS1 / blaCTX-M-55 / blaTEM-1B / blaLAP-2 / aac(3)-IId / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / floR / aph(3')-Ia - 1..267429 267429


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T201916 WP_000088046.1 NZ_CP074357:c214057-212732 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T201916 NZ_CP098926:c2459351-2459052 [Halomonas sp. MS1]
ATGCTCAGCTTCCGTATCACGCCAAGGGCAAGAGACGATTTAAAAAATATCGGCCGCTATACGGAGTGGCAATGGGGCAA
GAACCAACGAAATACCTATCTAAAGAATTTTGAAAAACGGTTTCACTGGCTCGCAGAAAATCCCCAGTTGGGAAAGCACC
GCTCGGATGTTAGCGAGGGTTATTACAGCTACTCTCAGGGCCAGCACGTTATCTTTTATCTTATCGGCCAGGAATGTATT
GAAATCATTGGCATTCCTCACAAAGAGATGGATATCGTTTCATATTTTCTGCCTGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT201916 WP_024136327.1 NZ_CP074357:c214355-214062 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT201916 NZ_CP098926:c2459599-2459351 [Halomonas sp. MS1]
ATGGCTACCACCAGTCTTAGCTTAGGGGAGCACTGGGAAGTCTTTATCAGAAACGAAGTCTCTAGCGGACGCTACGGCTC
GGCAAGTGAGGTTGTGCGTGATGCGTTACGTGCAATGGAAGAGCGCAAAAGCAAACTTGAAGCGCTTCGAACTCACTTGG
CCCAAGGTGCAGAGCAAGCAAGAACCGGTGAATTCGTGGATGATTTTTCGATGGATGCATTAATCAACGATTTGGATAGT
GAAACGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References