Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4395069..4395566 Replicon chromosome
Accession NZ_CP074356
Organism Escherichia coli strain XJ6.1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFQ18_RS20980 Protein ID WP_001173343.1
Coordinates 4395069..4395242 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFQ18_RS20985 Protein ID WP_000398619.1
Coordinates 4395270..4395566 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ18_RS20970 (4391739) 4391739..4393196 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
KFQ18_RS20975 (4393433) 4393433..4394950 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFQ18_RS20980 (4395069) 4395069..4395242 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFQ18_RS20985 (4395270) 4395270..4395566 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFQ18_RS20990 (4395793) 4395793..4396065 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFQ18_RS20995 (4396077) 4396077..4396307 - 231 WP_275877445.1 4Fe-4S mono-cluster protein YjdI -
KFQ18_RS21000 (4396488) 4396488..4398119 + 1632 WP_001216486.1 sensor histidine kinase -
KFQ18_RS21005 (4398116) 4398116..4398835 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201914 WP_001173343.1 NZ_CP074356:c4395242-4395069 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201914 NZ_CP098926:1326156-1326620 [Halomonas sp. MS1]
GTGAATGAGGTCAACGCTTACCGCTTGATCAAGCGCAAGTATCAGGACAACGCTTTCGATGGCGAAGGTGCACGCCTTTA
TGGCGGGCGCTGGAACAGCCCCGGTAAGGCCTGTGTGTATGTGGCAAGCGCCGAGTCGCTGGCGCTGCTTGAAATCATGG
TGCATCTGGAAAGCTATCAGTTGCTCAATGCCTATGCGCTGCTGCGCCTGACATTGCCGGCCGATAGCATTCTAGGTGTT
GGGGAAGCTGACCTACCGGACAACTGGCGGGAAGCACCTGCGCCAGCAGAAACTGCCGAGTTGGGCGACGGATGGCTAGA
CTCCGGCCACAGTCTGGCCTTGGCCATTCCCAGCGTGGTCGTGCCAAGAGAATTCAACTACATGCTCAATCCTGAGCATC
CCCTGTTTGATCAAGTCATCACGACGGCAGAGGCGTTGCCCTTCCAGCCCGACCCAAAACTTTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201914 WP_000398619.1 NZ_CP074356:c4395566-4395270 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201914 NZ_CP098926:1325710-1326159 [Halomonas sp. MS1]
ATGGATATGCACTTCACTCAATACCAGCCACCCAAAGCCTTGGCATCAGGTTTCTGGCACCAGATCGGCCTGCCTGCCAG
AGGCCGCAAGCTGCATGAGCTTTTGCATCAAGGCATTGAGTATAGGGTCTACCCCAGACTGGCTAAGGCGTCAGGTATCG
AACAGAAGGCGCTGGCAGGCTACGTCGATATTCCCCCGGCCACGCTGAGCCGCCGTGCTAAGTCGGGGCGCTTTAAGATG
GCGGAAAGCGACCGGCTATATCGCTTTGCCGAACTCTTCAAGGCGGCGCTAGACCTGTTTGAAGGCGACGTCGAGGGTGC
CCGCACCTGGCTGCTCAAGCCCAATACGGGTCTGGGCGGGCGCAGGCCCATCGAAATGAGCGCAACCTCTGCCGAGTATC
AAACGGTGTTGAACCTGATTGGGCGACTGGAGCACGGGGTTGCTACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References