Detailed information of TA system TA201913

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4204342..4205174
• Replicon: chromosome
• Accession: NZ_CP074356
• Organism: Escherichia coli strain XJ6.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: KFQ18_RS20030
• Protein ID: WP_000854765.1
• Coordinates: 4204342..4204716 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: KFQ18_RS20035
• Protein ID: WP_001295723.1
• Coordinates: 4204806..4205174 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ18_RS20010 (4199859)	4199859..4202750	-	2892	WP_000580534.1	SNF2-related protein	-
KFQ18_RS20015 (4203406)	4203406..4203555	-	150	Protein_3915	hypothetical protein	-
KFQ18_RS20020 (4203661)	4203661..4203837	-	177	WP_000839290.1	DUF957 domain-containing protein	-
KFQ18_RS20025 (4203854)	4203854..4204345	-	492	WP_000976842.1	DUF5983 family protein	-
KFQ18_RS20030 (4204342)	4204342..4204716	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
KFQ18_RS20035 (4204806)	4204806..4205174	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFQ18_RS20040 (4205337)	4205337..4205558	-	222	WP_000692345.1	DUF987 domain-containing protein	-
KFQ18_RS20045 (4205621)	4205621..4206097	-	477	WP_001186775.1	RadC family protein	-
KFQ18_RS20050 (4206113)	4206113..4206586	-	474	WP_000855059.1	antirestriction protein	-
KFQ18_RS20055 (4206928)	4206928..4207746	-	819	WP_001234652.1	DUF932 domain-containing protein	-
KFQ18_RS20060 (4207864)	4207864..4208059	-	196	Protein_3924	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T201913 WP_000854765.1 NZ_CP074356:c4204716-4204342 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T201913 NZ_CP098926:122674-123018 [Halomonas sp. MS1]
ATGTGGTCTATCGAGCAAACAGACACATTTGAAGCGTGGTTCTTCTCATTGGAAGAAATTGAACGTGAGAACGTTCTGGC
TTCTGTACTTCTTCTACGTGAACGAGGCCCGATGCTTTCAAGGCCGCACGCTGACACTGTAAACGGCTCGCAGCATTCCA
ACATGAAGGAATTGCGTGTTCAGTGCCAGGGACGTCCTATCAGAGTATTTTTTGCGTTTGATCCACGTCGCACAGGCATC
TTGCTATGCGCAGGCGATAAAGGTGGAAATGACAAGCGCTTCTATAATGAGATGATTCCAGTCGCCGATAAAGAATATAC
GGCTCACCTGGAAACACTAAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT201913 WP_001295723.1 NZ_CP074356:c4205174-4204806 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT201913 NZ_CP098926:123029-123331 [Halomonas sp. MS1]
ATGGCACGCAAACTGGAAGACTTGTTAGCAAAAGAAAAGCCAGAGATCGTTGCGGCAGCTGAGGCGAAAGCCAACGATAT
GTTATTCGAGATCAGGCTTGCCGAAATTCGTCAGCTAGCGGAGAAAACCCAAGCAGACATAGCTATTGCGATGGGAGTAA
AACAACCCACCGTCGCAGGACTTGAGAAAGTTGGCCAGGATATGCGCCTTAGTTCACTCAAACGCTACGTCGAAGCGCTA
GGCGGCCGAGTCCGTGTCGATATAGAACTTCCCGATGGAAGTCATCATGGCTTCCCTGTATAG

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52