Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4163223..4163799 Replicon chromosome
Accession NZ_CP074356
Organism Escherichia coli strain XJ6.1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFQ18_RS19830 Protein ID WP_001297643.1
Coordinates 4163512..4163799 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KFQ18_RS19825 Protein ID WP_000063160.1
Coordinates 4163223..4163525 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ18_RS19805 (4158914) 4158914..4159426 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
KFQ18_RS19810 (4159819) 4159819..4161969 + 2151 WP_275877439.1 pyruvate/proton symporter BtsT -
KFQ18_RS19815 (4162019) 4162019..4162222 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFQ18_RS19820 (4162233) 4162233..4163189 + 957 WP_001297640.1 GTPase -
KFQ18_RS19825 (4163223) 4163223..4163525 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KFQ18_RS19830 (4163512) 4163512..4163799 - 288 WP_001297643.1 BrnT family toxin Toxin
KFQ18_RS19835 (4163998) 4163998..4164694 - 697 Protein_3879 IS1 family transposase -
KFQ18_RS19840 (4165008) 4165008..4166240 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
KFQ18_RS19845 (4166281) 4166281..4167561 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4163998..4164207 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T201912 WP_001297643.1 NZ_CP074356:c4163799-4163512 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T201912 NZ_CP098836:c32257-32006 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT201912 WP_000063160.1 NZ_CP074356:c4163525-4163223 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT201912 NZ_CP098836:c32019-31792 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References