Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 94324..95947 Replicon plasmid unnamed1
Accession NZ_CP074355
Organism Escherichia coli strain XJ5.2

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag KFQ11_RS23285 Protein ID WP_000088046.1
Coordinates 94622..95947 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag KFQ11_RS23280 Protein ID WP_024136327.1
Coordinates 94324..94617 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ11_RS23250 (KFQ11_22970) 90196..91437 + 1242 WP_001053338.1 VWA domain-containing protein -
KFQ11_RS23255 91528..91659 + 132 WP_000374059.1 hypothetical protein -
KFQ11_RS23260 (KFQ11_22975) 92000..92281 - 282 WP_000077926.1 hypothetical protein -
KFQ11_RS23265 (KFQ11_22980) 92331..92522 - 192 WP_000398480.1 hypothetical protein -
KFQ11_RS23270 (KFQ11_22985) 92614..92955 - 342 WP_001151575.1 hypothetical protein -
KFQ11_RS23275 (KFQ11_22990) 93328..93720 + 393 WP_000341066.1 hypothetical protein -
KFQ11_RS23280 (KFQ11_22995) 94324..94617 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
KFQ11_RS23285 (KFQ11_23000) 94622..95947 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
KFQ11_RS23290 (KFQ11_23005) 96008..96214 + 207 WP_000134171.1 hypothetical protein -
KFQ11_RS23295 (KFQ11_23010) 96316..96726 + 411 WP_000985911.1 hypothetical protein -
KFQ11_RS23300 (KFQ11_23015) 96739..97554 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
KFQ11_RS23305 (KFQ11_23020) 97838..98535 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
KFQ11_RS23310 (KFQ11_23025) 98579..99244 - 666 WP_000490638.1 hypothetical protein -
KFQ11_RS23315 (KFQ11_23030) 99302..99682 - 381 WP_001426317.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-55 / aadA5 / sul2 / floR - 1..209150 209150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T201890 WP_000088046.1 NZ_CP074355:94622-95947 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T201890 NZ_CP098831:c4190622-4190338 [Salmonella enterica subsp. enterica serovar Indiana]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT201890 WP_024136327.1 NZ_CP074355:94324-94617 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT201890 NZ_CP098831:c4190854-4190612 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTACGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References