Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4169078..4169575 Replicon chromosome
Accession NZ_CP074354
Organism Escherichia coli strain XJ5.2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFQ11_RS20720 Protein ID WP_001173343.1
Coordinates 4169078..4169251 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFQ11_RS20725 Protein ID WP_000398619.1
Coordinates 4169279..4169575 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFQ11_RS20710 (4165748) 4165748..4167205 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
KFQ11_RS20715 (4167442) 4167442..4168959 + 1518 WP_001295087.1 lysine--tRNA ligase -
KFQ11_RS20720 (4169078) 4169078..4169251 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFQ11_RS20725 (4169279) 4169279..4169575 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFQ11_RS20730 (4169802) 4169802..4170074 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFQ11_RS20735 (4170086) 4170086..4170316 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFQ11_RS20740 (4170497) 4170497..4172128 + 1632 WP_001216477.1 sensor histidine kinase -
KFQ11_RS20745 (4172125) 4172125..4172844 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T201889 WP_001173343.1 NZ_CP074354:c4169251-4169078 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T201889 NZ_CP098831:3692822-3693073 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCGGGATACTTACAAAACCCACATGACCACCTATTACTCGCGCATCCCCAGCCTGCACCTTAAGGGCGACTGGCTGGC
AGAAGCGGGATTTGATACCGGGGCGAGCGTGACCGTGAAGATCTCCGAAGGGTGTTTGACTCTGATTGTGGAGACAGACG
AAGTGAGGGATCTACGGAGGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCACATTAAGGCGGGGGTATATGATGTGGTG
AACGGGAATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT201889 WP_000398619.1 NZ_CP074354:c4169575-4169279 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT201889 NZ_CP098831:c3693063-3692838 [Salmonella enterica subsp. enterica serovar Indiana]
TTCACCACATCATATACCCCCGCCTTAATGTGCTTCATCGACTTTTTCACCTGATAAAGCTCCCTCCGTAGATCCCTCAC
TTCGTCTGTCTCCACAATCAGAGTCAAACACCCTTCGGAGATCTTCACGGTCACGCTCGCCCCGGTATCAAATCCCGCTT
CTGCCAGCCAGTCGCCCTTAAGGTGCAGGCTGGGGATGCGCGAGTAATAGGTGGTCATGTGGGTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References