Detailed information of TA system TA201888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4070086..4070681
• Replicon: chromosome
• Accession: NZ_CP074354
• Organism: Escherichia coli strain XJ5.2

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: KFQ11_RS20195
• Protein ID: WP_089601988.1
• Coordinates: 4070086..4070436 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: U9Y2K1
• Locus tag: KFQ11_RS20200
• Protein ID: WP_001223208.1
• Coordinates: 4070430..4070681 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ11_RS20175 (4065535)	4065535..4066557	-	1023	WP_122992735.1	ABC transporter permease	-
KFQ11_RS20180 (4066571)	4066571..4068073	-	1503	WP_000210557.1	sugar ABC transporter ATP-binding protein	-
KFQ11_RS20185 (4068213)	4068213..4069169	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
KFQ11_RS20190 (4069479)	4069479..4070009	+	531	WP_000055072.1	inorganic diphosphatase	-
KFQ11_RS20195 (4070086)	4070086..4070436	-	351	WP_089601988.1	endoribonuclease toxin ChpB	Toxin
KFQ11_RS20200 (4070430)	4070430..4070681	-	252	WP_001223208.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
KFQ11_RS20205 (4070893)	4070893..4071234	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
KFQ11_RS20210 (4071237)	4071237..4075016	-	3780	WP_000060898.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12493.41 Da
• Isoelectric Point: 5.6219

>T201888 WP_089601988.1 NZ_CP074354:c4070436-4070086 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGITLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T201888 NZ_CP098831:3369538-3369756 [Salmonella enterica subsp. enterica serovar Indiana]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT201888 WP_001223208.1 NZ_CP074354:c4070681-4070430 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT201888 NZ_CP098831:3369136-3369510 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LQ26