Detailed information of TA system TA201887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3934703..3935279
• Replicon: chromosome
• Accession: NZ_CP074354
• Organism: Escherichia coli strain XJ5.2

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: D3GXZ2
• Locus tag: KFQ11_RS19530
• Protein ID: WP_001297643.1
• Coordinates: 3934992..3935279 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: KFQ11_RS19525
• Protein ID: WP_282318055.1
• Coordinates: 3934703..3935005 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ11_RS19510 (3931299)	3931299..3933449	+	2151	WP_282318054.1	pyruvate/proton symporter BtsT	-
KFQ11_RS19515 (3933499)	3933499..3933702	+	204	WP_000467859.1	YbdD/YjiX family protein	-
KFQ11_RS19520 (3933713)	3933713..3934669	+	957	WP_001297640.1	GTPase	-
KFQ11_RS19525 (3934703)	3934703..3935005	-	303	WP_282318055.1	BrnA antitoxin family protein	Antitoxin
KFQ11_RS19530 (3934992)	3934992..3935279	-	288	WP_001297643.1	BrnT family toxin	Toxin
KFQ11_RS19535 (3935478)	3935478..3936175	-	698	Protein_3824	IS1 family transposase	-
KFQ11_RS19540 (3936232)	3936232..3936327	+	96	Protein_3825	dienelactone hydrolase family protein	-
KFQ11_RS19545 (3936473)	3936473..3937705	+	1233	WP_024186283.1	multidrug efflux MFS transporter MdtM	-
KFQ11_RS19550 (3937746)	3937746..3939026	+	1281	WP_282318056.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11295.77 Da
• Isoelectric Point: 6.8821

>T201887 WP_001297643.1 NZ_CP074354:c3935279-3934992 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T201887 NZ_CP098831:2393375-2393659 [Salmonella enterica subsp. enterica serovar Indiana]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTACGGGAACGACTCGAAAACCCTCGGGTTCCTGCTTCGCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGGCACAGAAGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11582.09 Da
• Isoelectric Point: 9.4646

>AT201887 WP_282318055.1 NZ_CP074354:c3935005-3934703 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWFNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT201887 NZ_CP098831:2393137-2393385 [Salmonella enterica subsp. enterica serovar Indiana]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829GEV9