Detailed information of TA system TA201884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3442772..3443609
• Replicon: chromosome
• Accession: NZ_CP074354
• Organism: Escherichia coli strain XJ5.2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFQ11_RS17205
• Protein ID: WP_000227784.1
• Coordinates: 3443067..3443609 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFQ11_RS17200
• Protein ID: WP_001297137.1
• Coordinates: 3442772..3443083 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ11_RS17175 (3437792)	3437792..3438739	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFQ11_RS17180 (3438761)	3438761..3440752	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFQ11_RS17185 (3440742)	3440742..3441356	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFQ11_RS17190 (3441356)	3441356..3441685	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFQ11_RS17195 (3441697)	3441697..3442587	+	891	WP_097346802.1	heme o synthase	-
KFQ11_RS17200 (3442772)	3442772..3443083	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFQ11_RS17205 (3443067)	3443067..3443609	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFQ11_RS17210 (3443665)	3443665..3444600	-	936	WP_001490882.1	tetratricopeptide repeat protein	-
KFQ11_RS17215 (3445008)	3445008..3446372	+	1365	WP_282317769.1	MFS transporter	-
KFQ11_RS17220 (3446500)	3446500..3446991	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
KFQ11_RS17225 (3447159)	3447159..3448070	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T201884 WP_000227784.1 NZ_CP074354:3443067-3443609 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T201884 NZ_CP098831:823536-823934 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT201884 WP_001297137.1 NZ_CP074354:3442772-3443083 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT201884 NZ_CP098831:823309-823536 [Salmonella enterica subsp. enterica serovar Indiana]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3