Detailed information of TA system TA201871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 989634..990217
• Replicon: chromosome
• Accession: NZ_CP074354
• Organism: Escherichia coli strain XJ5.2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KFQ11_RS04990
• Protein ID: WP_089601808.1
• Coordinates: 989882..990217 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A161R357
• Locus tag: KFQ11_RS04985
• Protein ID: WP_040062758.1
• Coordinates: 989634..989882 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFQ11_RS04975 (985973)	985973..987274	+	1302	WP_000046826.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
KFQ11_RS04980 (987322)	987322..989556	+	2235	WP_000226803.1	GTP diphosphokinase	-
KFQ11_RS04985 (989634)	989634..989882	+	249	WP_040062758.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KFQ11_RS04990 (989882)	989882..990217	+	336	WP_089601808.1	endoribonuclease MazF	Toxin
KFQ11_RS04995 (990288)	990288..991079	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
KFQ11_RS05000 (991307)	991307..992944	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
KFQ11_RS05005 (993032)	993032..994330	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
KFQ11_RS05010 (994474)	994474..994667	+	194	Protein_982	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12142.09 Da
• Isoelectric Point: 8.2618

>T201871 WP_089601808.1 NZ_CP074354:989882-990217 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKSSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTLAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T201871 NZ_CP098829:717513-717896 [Salmonella enterica subsp. enterica serovar Indiana]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5972

>AT201871 WP_040062758.1 NZ_CP074354:989634-989882 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT201871 NZ_CP098829:717055-717423 [Salmonella enterica subsp. enterica serovar Indiana]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAATCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A161R357