Detailed information of TA system TA201849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 611235..611812
• Replicon: plasmid unnamed
• Accession: NZ_CP074351
• Organism: Pantoea dispersa strain YSD_J2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KFZ74_RS22930
• Protein ID: WP_058770345.1
• Coordinates: 611480..611812 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KFZ74_RS22925
• Protein ID: WP_238562162.1
• Coordinates: 611235..611480 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFZ74_RS22905 (KFZ74_22885)	606598..607551	+	954	WP_127835008.1	carbohydrate kinase family protein	-
KFZ74_RS22910 (KFZ74_22890)	607555..608637	+	1083	WP_238562160.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
KFZ74_RS22915 (KFZ74_22895)	608756..610165	-	1410	WP_238562161.1	MFS transporter	-
KFZ74_RS22920 (KFZ74_22900)	610281..611135	+	855	WP_211724536.1	helix-turn-helix transcriptional regulator	-
KFZ74_RS22925 (KFZ74_22905)	611235..611480	+	246	WP_238562162.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KFZ74_RS22930 (KFZ74_22910)	611480..611812	+	333	WP_058770345.1	endoribonuclease MazF	Toxin
KFZ74_RS22935 (KFZ74_22915)	611918..612091	+	174	WP_021508895.1	hypothetical protein	-
KFZ74_RS22940 (KFZ74_22920)	612121..612468	+	348	WP_021508894.1	hypothetical protein	-
KFZ74_RS22945 (KFZ74_22925)	612508..613701	-	1194	WP_021508893.1	zinc-dependent alcohol dehydrogenase	-
KFZ74_RS22950 (KFZ74_22930)	613965..614672	+	708	WP_021508892.1	phage antirepressor KilAC domain-containing protein	-
KFZ74_RS22955 (KFZ74_22935)	614707..614922	-	216	WP_021508891.1	hypothetical protein	-
KFZ74_RS22960 (KFZ74_22940)	615064..616245	-	1182	WP_238562163.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN	1..710238	710238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• Antitoxin PemI-like (4-80)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12009.84 Da
• Isoelectric Point: 7.8855

>T201849 WP_058770345.1 NZ_CP074351:611480-611812 [Pantoea dispersa]
MVSRFVPDAGDLIWLDFDPTLGHEQGGHRPAVVLSPFAYNNKVGMLLCVPCTTQVKGYPFEVSLTGSRDSVALADQVTSI
DWRARKVVKKSHVTAEELSEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T201849 NZ_CP098795:3281073-3281462 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAGTAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGTAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8971.31 Da
• Isoelectric Point: 4.5580

>AT201849 WP_238562162.1 NZ_CP074351:611235-611480 [Pantoea dispersa]
MTTVTLKKWGNSPSIRIPSSVMQSASLCVDDKLELSVEDGKLILVPLREKVYSLDALLDEITDDNVHDKIDFGPSVGKER
I

Nucleotide

• Length: 246 bp

>AT201849 NZ_CP098795:3280809-3281066 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCATTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA