Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | pmenTA/darT(toxin) |
| Location | 5250357..5252086 | Replicon | chromosome |
| Accession | NZ_CP074349 | ||
| Organism | Pseudomonas cichorii strain DSM 50259 | ||
Toxin (Protein)
| Gene name | pmenT | Uniprot ID | A0A3M4WAB2 |
| Locus tag | KGD89_RS22385 | Protein ID | WP_025261997.1 |
| Coordinates | 5250357..5251001 (+) | Length | 215 a.a. |
Antitoxin (Protein)
| Gene name | pmenA | Uniprot ID | A0A3M4WAC2 |
| Locus tag | KGD89_RS22390 | Protein ID | WP_025261998.1 |
| Coordinates | 5250998..5252086 (+) | Length | 363 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KGD89_RS22360 (KGD89_22355) | 5245810..5246457 | + | 648 | WP_025261992.1 | DedA family protein | - |
| KGD89_RS22365 (KGD89_22360) | 5246461..5247273 | + | 813 | WP_025261993.1 | zinc-dependent peptidase | - |
| KGD89_RS22370 (KGD89_22365) | 5247378..5247905 | + | 528 | WP_025261994.1 | inorganic diphosphatase | - |
| KGD89_RS22375 (KGD89_22370) | 5248250..5248981 | + | 732 | WP_025261995.1 | XRE family transcriptional regulator | - |
| KGD89_RS26075 | 5249450..5249605 | + | 156 | Protein_4420 | restriction endonuclease | - |
| KGD89_RS22380 (KGD89_22375) | 5249779..5250360 | + | 582 | WP_025261996.1 | DarT ssDNA thymidine ADP-ribosyltransferase family protein | - |
| KGD89_RS22385 (KGD89_22380) | 5250357..5251001 | + | 645 | WP_025261997.1 | DUF4433 domain-containing protein | Toxin |
| KGD89_RS22390 (KGD89_22385) | 5250998..5252086 | + | 1089 | WP_025261998.1 | macro domain-containing protein | Antitoxin |
| KGD89_RS22395 (KGD89_22390) | 5252229..5252552 | - | 324 | WP_236250086.1 | transcriptional regulator | - |
| KGD89_RS26080 | 5252849..5253220 | - | 372 | WP_025262000.1 | hypothetical protein | - |
| KGD89_RS26185 | 5253371..5255050 | - | 1680 | WP_264302371.1 | calcium-binding protein | - |
| KGD89_RS26190 | 5255207..5255431 | - | 225 | Protein_4427 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 5249779..5264202 | 14423 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 215 a.a. Molecular weight: 24844.35 Da Isoelectric Point: 6.6571
>T201838 WP_025261997.1 NZ_CP074349:5250357-5251001 [Pseudomonas cichorii]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNADLIDKRRSRDVPIAPMGVLADYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLHRVEQLGLPFVFTNAHAYPDWTNYYSDLAHLSEIDWPILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYTDAMKERIEQDVVARGLTLSVHARPGWYFQ
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCASSDVQAPQYVNIGNADLIDKRRSRDVPIAPMGVLADYVPFYFTPFSV
MMKNIHSGWSVQQRSNDEIVILVSSLHRVEQLGLPFVFTNAHAYPDWTNYYSDLAHLSEIDWPILQRRDFKRDPDDPRKM
ERYQAEALIHHHLPITGLLGIMCYTDAMKERIEQDVVARGLTLSVHARPGWYFQ
Download Length: 645 bp
>T201838 NZ_CP098778:c2840121-2839768 [Enterobacter hormaechei]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA
Antitoxin
Download Length: 363 a.a. Molecular weight: 40747.67 Da Isoelectric Point: 6.3146
>AT201838 WP_025261998.1 NZ_CP074349:5250998-5252086 [Pseudomonas cichorii]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAGEVETGKVHVTAVNELEGPRWIVNFPTK
RHWRSPSQMQWITEGLHDLRRFLIENHVKSVAVPPLGAGNGGLKWPEVREQIVDALSDLDVDVLVFEPSSQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIKNLPGTKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDADISDVIWFDQDRKAFLQTYLKTEAREYSQALELTAELIDGFESPFGMELLATVDWLLSQEEVSPTVPTVREA
LRHWDGGTGAAARKSKLFDDQAIDIALKRLTSSSFSSAVVNC
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAENYRLYSAACKAGEVETGKVHVTAVNELEGPRWIVNFPTK
RHWRSPSQMQWITEGLHDLRRFLIENHVKSVAVPPLGAGNGGLKWPEVREQIVDALSDLDVDVLVFEPSSQYLNVAKRSG
VEKLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIKNLPGTKNPLDLKFVAHKYGPYANRLEHLLDNLDGSYLH
CDKRISDADISDVIWFDQDRKAFLQTYLKTEAREYSQALELTAELIDGFESPFGMELLATVDWLLSQEEVSPTVPTVREA
LRHWDGGTGAAARKSKLFDDQAIDIALKRLTSSSFSSAVVNC
Download Length: 1089 bp
>AT201838 NZ_CP098778:c2839763-2839461 [Enterobacter hormaechei]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3M4WAB2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3M4WAC2 |